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Automated Robotic Liquid Handling Assembly of Modular DNA Devices
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Automation of 3D digital rolling circle amplification using a 3D-printed liquid handler.

Suyeon Shin1, Hyo Geun Yun1, Haerim Chung2

  • 1Department of Electronic Engineering, Hanyang University, Seoul, 04763, Republic of Korea.

Biosensors & Bioelectronics
|June 21, 2024
PubMed
Summary

This study introduces a cost-effective, 3D-printed liquid handler using a fluidic manifold, simplifying automation for biochemical assays. This innovation enhances throughput and reproducibility in laboratory workflows, particularly for complex assays like 4D digital rolling circle amplification.

Keywords:
3D digital rolling circle amplification3D printingLeukemia diagnosisLiquid handler

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Area of Science:

  • Biomedical Engineering
  • Biochemistry
  • Laboratory Automation

Background:

  • Automated liquid handling is crucial for improving throughput and reproducibility in biochemical assays.
  • High costs and complexity of robotic systems often limit the adoption of laboratory automation.

Purpose of the Study:

  • To develop an accessible and cost-effective automated liquid handling system.
  • To demonstrate the utility of a 3D-printed fluidic manifold for biochemical assay automation.

Main Methods:

  • Designed and 3D-printed a dispensing and aspirating (DA) device, a fluidic manifold with parallelized multi-pipette structures.
  • Engineered a custom liquid handler for 3D digital rolling circle amplification (4DRCA).
  • Validated the system for automating sample preparation for simultaneous oncogenic marker analysis in leukemia cells.

Main Results:

  • The 3D-printed DA device successfully automated complex liquid handling steps for 4DRCA.
  • The system enabled precise reagent supply and removal, enhancing assay reproducibility.
  • Demonstrated efficacy in preparing clinical samples for simultaneous in situ analysis of protein and transcript markers.

Conclusions:

  • 3D printing offers a versatile and affordable approach to creating custom liquid handlers.
  • The developed fluidic manifold-based system provides an effective solution for high-throughput, reproducible biochemical assay automation.
  • This technology can significantly lower barriers to adopting automation in research and clinical laboratories.