Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hybrid biofabrication of multilayered 3D neuronal networks with structural and functional interlayer connectivity.

Biosensors & bioelectronics·2025
Same author

Connectivity and network burst properties of in-vitro neuronal networks induced by a clustered structure with alginate hydrogel patterning.

Biomedical engineering letters·2023
Same author

Enhancement of Thermoplasmonic Neural Modulation Using a Gold Nanorod-Immobilized Polydopamine Film.

ACS applied materials & interfaces·2022
Same author

Optical recording of neural responses to gold-nanorod mediated photothermal neural inhibition.

Journal of neuroscience methods·2022
Same author

Neurons-on-a-Chip: <i>In Vitro</i> NeuroTools.

Molecules and cells·2022
Same author

Development of the micro-patterned 3D neuronal-hydrogel model using soft-lithography for study a 3D neural network on a microelectrode array<sup></sup>.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2021
Same journal

Propylene carbonate-PVDF-HFP/MXene-based self-powered biosensor for auxiliary detection of salivary exosomal miRNA-155 in pediatric asthma.

Biosensors & bioelectronics·2026
Same journal

Nanostructured zinc-coordination supraparticles on cellulose fibers: A 3D-Printed μ-FAD integrated smartphone platform for multiplexed salivary metabolic monitoring.

Biosensors & bioelectronics·2026
Same journal

Reliable biomarker monitoring at microneedle aptamer biosensors using a dual-frequency ratiometric approach: Overcoming signal drifts.

Biosensors & bioelectronics·2026
Same journal

Interfacial structure-modified nanozyme drives single-receptor-single-reaction-unit multichannel sensor array for pesticide discrimination.

Biosensors & bioelectronics·2026
Same journal

A real-time 5-hydroxytryptamine monitoring system applicable both in vitro and in vivo.

Biosensors & bioelectronics·2026
Same journal

Recent developments of textile-based triboelectric nanogenerators for smart sports applications.

Biosensors & bioelectronics·2026
See all related articles

Related Experiment Video

Updated: Jun 23, 2025

Author Spotlight: Advancing Large-Scale Neural Dynamics Through HD-MEA Technology
09:44

Author Spotlight: Advancing Large-Scale Neural Dynamics Through HD-MEA Technology

Published on: March 8, 2024

4.7K

A 3D neuronal network read-out interface with high recording performance using a neuronal cluster patterning on a

Dongjo Yoon1, Yoonkey Nam1

  • 1Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea.

Biosensors & Bioelectronics
|June 21, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces a novel neuronal cluster interface to improve signal measurement in 3D neuronal networks using microelectrode arrays (MEAs). The interface enhances active channel ratio and signal-to-noise ratio for reliable 3D network activity analysis.

Keywords:
Cell cultureElectrophysiologyHydrogelMicroelectrode arrayNeural interfaceNeuron

More Related Videos

Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model
09:47

Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model

Published on: October 18, 2015

10.0K
Author Spotlight: Unraveling Seizure Dynamics and Novel Therapeutics for Status Epilepticus Using CMOS High-Density Microelectrode Array Systems
06:28

Author Spotlight: Unraveling Seizure Dynamics and Novel Therapeutics for Status Epilepticus Using CMOS High-Density Microelectrode Array Systems

Published on: September 27, 2024

2.3K

Related Experiment Videos

Last Updated: Jun 23, 2025

Author Spotlight: Advancing Large-Scale Neural Dynamics Through HD-MEA Technology
09:44

Author Spotlight: Advancing Large-Scale Neural Dynamics Through HD-MEA Technology

Published on: March 8, 2024

4.7K
Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model
09:47

Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model

Published on: October 18, 2015

10.0K
Author Spotlight: Unraveling Seizure Dynamics and Novel Therapeutics for Status Epilepticus Using CMOS High-Density Microelectrode Array Systems
06:28

Author Spotlight: Unraveling Seizure Dynamics and Novel Therapeutics for Status Epilepticus Using CMOS High-Density Microelectrode Array Systems

Published on: September 27, 2024

2.3K

Area of Science:

  • Neuroscience
  • Biomedical Engineering
  • Materials Science

Background:

  • In vitro three-dimensional (3D) neuronal network models are advancing.
  • Measuring activity in 3D neuronal networks using 2D microelectrode arrays (MEAs) faces challenges with unstable physical coupling.
  • Improved methods are needed for reliable signal transduction from 3D networks to MEAs.

Purpose of the Study:

  • To develop a neuronal cluster interface for enhancing the active channel ratio of commercial 2D MEAs.
  • To enable reliable measurement and stimulation of 3D neuronal network activity.
  • To facilitate the study of functional activity in various 3D neuronal network models.

Main Methods:

  • Patterning neuronal clusters on microelectrodes to act as signal mediators.
  • Utilizing these neuronal clusters to bridge the gap between 3D networks and 2D MEAs.
  • Confirming enhanced active channel ratio and signal-to-noise ratio (SNR) for 3D network recording and stimulation over one month.

Main Results:

  • The neuronal cluster interface significantly improved the active channel ratio and SNR for 3D network recording and stimulation.
  • The interface achieved functional connectivity with 3D networks, enabling measurement without altering activity characteristics.
  • Demonstrated the interface's utility in analyzing 3D vs. 2D network dynamics and constructing 3D clustered networks.

Conclusions:

  • The proposed neuronal cluster interface offers a reliable method for measuring 3D neuronal network activity using commercial 2D MEAs.
  • This approach enhances signal transduction, improving the study of complex neural network dynamics.
  • The interface has broad applications for advancing research in 3D neuronal network models.