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Updated: Jul 28, 2026

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
Published on: March 14, 2021
Is there a correlation between membrane phospholipid metabolism and cell division?
Abstract:
The specificity of lipid/protein interactions in bacterial membranes is based on the diversity and variability of whole phospholipid molecules and of their polar and non-polar moieties. Of particular interest is the synthesis and turnover of anionic phospholipids facilitating variations in the phosphatidylglycerol (PG)/diphosphatidylglycerol (DPG) ratio in correlation with cell growth and division. Accumulation of DPG increases the anionic character of the cell membrane which is known to hinder the septation process. Consequently, the decrease in PG/DPG observed in stationary and in penicillin- or UV-inhibited non-dividing bacteria can be explained in this manner. On the other hand, the presence of lysophosphatidylethanolamine observed in the chain-forming mutant envC PM61 of Escherichia coli, and resulting from an anomaly in the deacylation-reacylation cycle, appears to be an indirect consequence rather than the cause of septation deficiency. Variations in the ratio of saturated/unsaturated fatty acids in phospholipids are also correlated with the physiological state of the cell. Hence, the levels of fluid and non-fluid phospholipids, their polymorphism and interaction with membrane proteins appear to be implicated in cell wall modelling. Future work will show how all these correlations function on the genetic and molecular levels.
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