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Updated: Jun 23, 2025
An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
Published on: April 5, 2018
Machine learning prediction of prime editing efficiency across diverse chromatin contexts
Nicolas Mathis1, Ahmed Allam2, András Tálas1
1Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.
None:
The success of prime editing depends on the prime editing guide RNA (pegRNA) design and target locus. Here, we developed machine learning models that reliably predict prime editing efficiency. PRIDICT2.0 assesses the performance of pegRNAs for all edit types up to 15 bp in length in mismatch repair-deficient and mismatch repair-proficient cell lines and in vivo in primary cells. With ePRIDICT, we further developed a model that quantifies how local chromatin environments impact prime editing rates.
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