Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Positron Emission Tomography01:29

Positron Emission Tomography

Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body being...
Radiological Investigation III: Pulmonary Angiogram and PET Scan01:13

Radiological Investigation III: Pulmonary Angiogram and PET Scan

Radiological investigations are paramount in the diagnosis and management of various pulmonary diseases. Two essential investigations are the Pulmonary Angiogram and the Positron Emission Tomography (PET) Scan.
Pulmonary Angiogram
A Pulmonary Angiogram is an invasive procedure involving injecting a contrast medium through a catheter threaded into the pulmonary artery or the right side of the heart to visualize the pulmonary vasculature. Computed Tomography (CT) scans have mainly replaced this...
Imaging Studies II: Positron Emission Tomography and Scintigraphy01:25

Imaging Studies II: Positron Emission Tomography and Scintigraphy

Positron Emission Tomography (PET) is a medical imaging technique that provides crucial insights into the body's physiological functions at a molecular level. It is an indispensable resource for diagnosing, staging, and monitoring various illnesses, notably cancer, neurological disorders, and cardiovascular conditions.
Fundamental Principles of PET

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From Ostrich to Mouse to Human: Translation of the Functional Liver PET Tracer [<sup>68</sup>Ga]Ga-TEoS-DAZA.

Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2026
Same author

Cortical Auditory Neural Responses to Speech and Communicative Functioning in Normocephalic Children With Prenatal Exposure to the Zika Virus.

Ear and hearing·2026
Same author

Development and <i>in vivo</i> evaluation of <sup>18</sup>F-labeled PET tracers covalently targeting KRAS-G12C for noninvasive cancer diagnosis and therapy monitoring.

Theranostics·2026
Same author

Image-derived input functions for [18F]LW223 and [18F]SynVesT-1 PET in the rodent determined using an autoencoder (IDIF-AE).

Physics in medicine and biology·2026
Same author

Comparative analysis of bypass vs. stent for coronary revascularization using an ex-vivo organ care system in an animal model.

Scientific reports·2026
Same author

Specific imaging of bacterial infection: a translational approach using positron emission tomography and gallium-68-labeled maltohexaose.

Theranostics·2026

Related Experiment Video

Updated: Jul 21, 2026

Analysis of 18FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates
10:04

Analysis of 18FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates

Published on: September 5, 2017

18.8K

Optimizing SUV Analysis: A Multicenter Study on Preclinical FDG-PET/CT Highlights the Impact of Standardization.

Claudia Kuntner1,2, Carlos Alcaide3, Dimitris Anestis4

  • 1Department of Biomedical Imaging and Image-Guided Therapy, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Vienna, Austria. claudia.kuntner@meduniwien.ac.at.

Molecular Imaging and Biology
|June 21, 2024
PubMed
Summary

Standardizing preclinical imaging analysis improves data reproducibility. A defined protocol for defining volumes of interest (VOIs) reduced variability in standard uptake values (SUVmean and SUVmax) across multiple organs.

Keywords:
Image analysisMulticenterPET/CTPreclinical imagingReproducibility

More Related Videos

Whole-body PET/MRI of Pediatric Patients: The Details That Matter
10:02

Whole-body PET/MRI of Pediatric Patients: The Details That Matter

Published on: December 19, 2017

14.6K
Semi-quantitative Assessment Using [18F]FDG Tracer in Patients with Severe Brain Injury
09:58

Semi-quantitative Assessment Using [18F]FDG Tracer in Patients with Severe Brain Injury

Published on: November 9, 2018

7.6K

Related Experiment Videos

Last Updated: Jul 21, 2026

Analysis of 18FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates
10:04

Analysis of 18FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates

Published on: September 5, 2017

18.8K
Whole-body PET/MRI of Pediatric Patients: The Details That Matter
10:02

Whole-body PET/MRI of Pediatric Patients: The Details That Matter

Published on: December 19, 2017

14.6K
Semi-quantitative Assessment Using [18F]FDG Tracer in Patients with Severe Brain Injury
09:58

Semi-quantitative Assessment Using [18F]FDG Tracer in Patients with Severe Brain Injury

Published on: November 9, 2018

7.6K

Area of Science:

  • Preclinical imaging
  • Nuclear medicine
  • Radiochemistry

Background:

  • Standardized methods for defining volumes of interest (VOIs) are lacking in preclinical imaging.
  • This variability impacts the reproducibility of data, particularly standard uptake values (SUVmean and SUVmax).
  • Translational potential of preclinical imaging is hindered by inconsistent analysis protocols.

Purpose of the Study:

  • To assess interobserver variability in VOI sizing and SUV measurements using [18F]FDG-PET and PET/CT datasets.
  • To evaluate the impact of a standardized analysis protocol on data comparability.
  • To determine the effect of observer experience (beginners vs. experts) on variability.

Main Methods:

  • 12 observers (4 beginners, 8 experts) analyzed identical preclinical [18F]FDG-PET and PET/CT datasets.
  • Initial analysis followed individual default protocols.
  • A standardized protocol detailing VOI size and position was introduced, followed by reanalysis.

Main Results:

  • Significant interobserver variability in SUVmean and SUVmax was observed without a standardized protocol.
  • Coregistration of CT images with PET images offered limited improvement in comparability.
  • The standardized protocol significantly reduced interobserver variability and enhanced comparability of SUV values.

Conclusions:

  • A standardized protocol for VOI definition improves comparability of SUV measurements in preclinical imaging.
  • The protocol was particularly beneficial for less experienced observers, enhancing data reliability.
  • Incorporating a VOI template could further improve consistency in preclinical imaging analyses.