Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mavacamten shows broad benefit in human and mouse models of MYBPC3-related hypertrophic cardiomyopathy.

Nature cardiovascular research·2026
Same author

Grape Seed Proanthocyanidins Enhance Time-Dependent HO-1 Activation and Improve Redox Homeostasis in Obesity-Induced Hepatic Dysfunction.

Antioxidants (Basel, Switzerland)·2026
Same author

OMA1 protects from liver injury and tumorigenesis during aging by controlling hepatic immunogenicity.

The EMBO journal·2026
Same author

Mitochondria directly interact with the nuclear pore complex.

Nature·2026
Same author

Pro-regenerative fingerprints identified in a sub-population of adult mouse cardiomyocytes by integrative single-cell proteomics.

Genome biology·2026
Same author

Small molecule inhibition of voltage dependent anion channel 1 reroutes mitochondrial metabolite flux.

Molecules and cells·2026

Related Experiment Video

Updated: Jun 23, 2025

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
06:13

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

3.9K

A Sample Preparation Procedure for Isobaric Labeling-Based Single-Cell Proteomics.

Consuelo Marín-Vicente1,2, Enrique Calvo3,4, José Manuel Rodríguez3

  • 1Cardiovascular Proteomics Group, Spanish National Centre for Cardiovascular Research (CNIC), Madrid, Spain. cmarin@cnic.es.

Methods in Molecular Biology (Clifton, N.J.)
|June 21, 2024
PubMed
Summary

This study presents a method for single-cell proteomics using mass spectrometry. The protocol enhances sensitivity for analyzing limited biological samples, including difficult-to-isolate cells.

Keywords:
Mass spectrometryMultiplex sample preparation strategyOrbitrap Eclipse mass spectrometerSingle-cell proteomicsTandem mass tagTribrid mass spectrometer

More Related Videos

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

11.9K
Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
08:29

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

6.1K

Related Experiment Videos

Last Updated: Jun 23, 2025

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
06:13

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

3.9K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

11.9K
Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
08:29

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

6.1K

Area of Science:

  • Proteomics
  • Mass Spectrometry
  • Cell Biology

Background:

  • Traditional mass spectrometry-based proteomics requires substantial input material, limiting its application.
  • Single-cell proteomics demands ultra-high sensitivity due to minute sample quantities.
  • Existing methods face challenges in analyzing rare or difficult-to-isolate cell types.

Purpose of the Study:

  • To establish a general workflow for preparing cells for single-cell mass spectrometry-based proteomics.
  • To adapt isobaric labeling strategies with a carrier proteome to overcome sensitivity limitations.
  • To provide a protocol applicable to manually isolated cells, particularly large cells like cardiomyocytes.

Main Methods:

  • Utilizing isobaric labeling-based multiplex strategies.
  • Incorporating a carrier proteome to enhance signal detection.
  • Developing a general cell preparation workflow for mass spectrometry analysis.
  • Adapting the protocol for manual cell isolation techniques.

Main Results:

  • A robust protocol for single-cell mass spectrometry-based proteomics is demonstrated.
  • The method effectively addresses sensitivity limitations inherent in single-cell analysis.
  • The workflow is adaptable for challenging cell types, including those not amenable to standard sorting.

Conclusions:

  • The presented protocol significantly advances the feasibility of single-cell proteomics.
  • This approach broadens the scope of mass spectrometry applications in cell biology research.
  • The method offers a valuable tool for analyzing precious or rare cell populations.