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Updated: Jun 23, 2025

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CRISPR/Cas9-mediated knockout strategies for enhancing immunotherapy in breast cancer

Chenchen Xu1

  • 1Department of Gynecology and Obstetrics, Changzhou Maternal and Child Health Care Hospital, Changzhou Medical Center, Nanjing Medical University, Changzhou, 213000, China. LinLin41539@gmail.com.

Naunyn-Schmiedeberg'S Archives of Pharmacology
|June 22, 2024
PubMed

Insights

Combining CRISPR/Cas9 gene knockout with immunotherapy offers a novel approach to enhance breast cancer treatment by targeting cancer cells and boosting immune response. This strategy holds promise for revolutionizing therapy, though safety requires careful evaluation.

Area of Science:

  • Oncology
  • Genetics
  • Immunology

Background:

  • Breast cancer presents significant treatment challenges due to its genetic complexity and mortality rates.
  • Current immunotherapies show promise but can be limited by cancer cell evasion mechanisms.

Purpose of the Study:

  • To explore the synergistic potential of combining CRISPR/Cas9 gene knockout with immunotherapy for enhanced breast cancer treatment.
  • To review how gene knockout can overcome immune evasion in breast cancer.

Main Methods:

  • Review of current literature on CRISPR/Cas9 gene editing applications in oncology.
  • Analysis of immunotherapeutic strategies for breast cancer.
  • Exploration of gene knockout targets to enhance immune system recognition of cancer cells.

Main Results:

  • CRISPR/Cas9 gene knockout offers precise genetic alteration to target cancer cells.
  • Integration of CRISPR/Cas9 and immunotherapy may augment treatment efficacy by disabling immune evasion pathways.
  • Potential for minimizing off-target effects through targeted gene editing.

Conclusions:

  • The combination of CRISPR/Cas9-mediated gene knockout and immunotherapy represents a promising strategy for advancing breast cancer treatment.
  • Further research is needed to optimize these integrated approaches and address safety considerations like off-target effects and immune responses.
Keywords:
Breast cancerCRISPR/Cas9Cancer researchImmune responseImmunotherapyKnockout strategiesOff-target effectsPrecision medicineTargeted genetic changes

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