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Updated: Jun 23, 2025

Author Spotlight: Enhancing Lipid Nanoparticle Formation Through Turbulent Mixing in Confined Geometries
Published on: August 23, 2024
Lipid-Based Nanoparticles for RNA Delivery
Xiomara Calderón-Colón1, Richard Egan2
1Asymmetric Operations Sector, Applied Biological Sciences, The Johns Hopkins University Applied Physics Laboratory, Laurel, MD, USA. Xiomara.Calderon-Colon@jhuapl.edu.
Abstract:
Various nanoparticle-based delivery systems have been developed for the encapsulation and protection of active cargoes. Lipid nanoparticles represent one of the most widely used nanoparticle-based delivery systems for in vitro and in vivo applications, especially for the delivery of ribonucleic acid (RNA). In this chapter, a simple bulk mixing method for the encapsulation of RNA is described along with characterization techniques for measuring encapsulation efficiency and nanoparticle physicochemical properties.
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