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Updated: Jun 23, 2025

Author Spotlight: Enhancing Lipid Nanoparticle Formation Through Turbulent Mixing in Confined Geometries

Author Spotlight: Enhancing Lipid Nanoparticle Formation Through Turbulent Mixing in Confined Geometries

Published on: August 23, 2024

Lipid-Based Nanoparticles for RNA Delivery.

Xiomara Calderón-Colón¹, Richard Egan²

¹Asymmetric Operations Sector, Applied Biological Sciences, The Johns Hopkins University Applied Physics Laboratory, Laurel, MD, USA. Xiomara.Calderon-Colon@jhuapl.edu.

Methods in Molecular Biology (Clifton, N.J.)

|June 22, 2024

Summary

This summary is machine-generated.

This chapter details a simple bulk mixing method for encapsulating ribonucleic acid (RNA) within lipid nanoparticles. It also covers essential characterization techniques to assess RNA encapsulation efficiency and nanoparticle properties for delivery applications.

Keywords:

Bulk mixing method Delivery Lipid nanoparticles Particle size Physicochemical properties Polydispersity index Ribonucleic acid Zeta potential

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Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

Area of Science:

Nanotechnology
Biomedical Engineering
Drug Delivery Systems

Background:

Nanoparticle-based systems are crucial for protecting and delivering active molecules.
Lipid nanoparticles (LNPs) are a prominent choice for in vitro and in vivo applications.
Effective delivery of ribonucleic acid (RNA) remains a key challenge in therapeutics.

Purpose of the Study:

To describe a straightforward bulk mixing method for RNA encapsulation in lipid nanoparticles.
To present characterization techniques for evaluating RNA encapsulation efficiency.
To outline methods for assessing the physicochemical properties of resulting nanoparticles.

Main Methods:

Implementation of a simple bulk mixing technique for RNA encapsulation.
Utilization of specific assays to determine encapsulation efficiency.
Application of physicochemical characterization methods for nanoparticle analysis.

Main Results:

Successful encapsulation of RNA using the described bulk mixing method.
Quantification of encapsulation efficiency achieved through the applied techniques.
Characterization of key physicochemical properties of the RNA-loaded lipid nanoparticles.

Conclusions:

The presented bulk mixing method offers a simple approach for RNA encapsulation in lipid nanoparticles.
Characterization techniques are vital for validating the quality and performance of these delivery systems.
This method and characterization approach support the development of RNA-based therapeutics.