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Updated: Jun 23, 2025
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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
27.9K
Techniques, procedures, and applications in microbiome analysis
Adriana González1, Asier Fullaondo1, Adrián Odriozola1
1Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country (UPV/EHU), Leioa, Spain.
Advances in Genetics
|June 22, 2024
Summary
Modern DNA sequencing and multi-omics reveal the microbiome
Area of Science:
- Microbiology
- Genomics
- Bioinformatics
Background:
- Historically, microbiota research relied on limited bacteriological culture techniques.
- Recent advancements in DNA sequencing and multi-omics have revolutionized understanding of microbial communities.
Purpose of the Study:
- To provide a comprehensive overview of current microbiome research methodologies.
- To summarize key concepts, experimental techniques, and data analysis in the field.
Main Methods:
- DNA sequencing technologies
- Multi-omics approaches
- Bioinformatics analysis
- Sample collection protocols
Main Results:
- Microbiome is integral to host biology, influencing development, physiology, reproduction, and evolution.
- Advanced techniques offer deeper insights into microbial community functions and host interactions.
Conclusions:
- The microbiome plays a crucial role in host health and evolution.
- This chapter serves as a guide for researchers entering the field of microbiome studies.
