[Cultivation of reaggregated brain cells in rapidly rotating mini-rollers]

An original method of cultivation of reaggregated brain cells with the aid of high-speed portable mini-rollers is described. The mini-roller consists of parallel rollers and an electric motor rotating the flasks at a speed of 60 to 70 rpm. The Moscona technique was used for preparing brain cell suspensions. During cultivation of the suspension of dissociated cells in high-speed mini-rollers, reaggregates with an internal organotypic structure were obtained. The method suggested provides stable and reproducible results.