Related Experiment Video

Updated: Jun 23, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.6K

Silencing LINC00987 ameliorates adriamycin resistance of acute myeloid leukemia via miR-4458/HMGA2 axis

Yue Liu1, Xiao-Ya Zhu2, Li-Li Liao3

  • 1Institute of Biomedicine, Department of Cellular Biology, Jinan University, No. 601 Huangpu Ave West, Shipai Street, Tianhe District, Guangzhou, Guangdong, 510632, China.

Biology Direct
|June 23, 2024
PubMed
Abstract

Insights

LINC00987 promotes Adriamycin resistance in acute myeloid leukemia (AML) by sponging miR-4458 and upregulating HMGA2. Downregulating LINC00987 may offer a new therapeutic strategy for chemoresistant AML.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genetics

Background:

  • Acute myeloid leukemia (AML) frequently develops drug resistance, leading to poor patient outcomes.
  • Long non-coding RNAs (lincRNAs) are increasingly recognized for their role in chemoresistance within AML.
  • The specific function of lincRNAs in Adriamycin (ADR) resistance in AML remains largely unknown.

Purpose of the Study:

  • To investigate the role of LINC00987 in Adriamycin (ADR) resistance in acute myeloid leukemia (AML) cells.
  • To elucidate the molecular mechanism by which LINC00987 influences chemoresistance in AML.

Main Methods:

  • Construction of ADR-resistant AML cell lines.
  • Quantification of LINC00987, miRNA, and HMGA2 mRNA expression via qRT-PCR.
  • Assessment of P-GP, BCRP, and HMGA2 protein levels using Western blot.
  • Evaluation of cell proliferation, IC50, colony formation, apoptosis, and xenograft tumor growth.

Main Results:

  • LINC00987 expression is elevated in AML patients and correlates with poorer survival.
  • LINC00987 expression significantly increases in ADR-resistant AML cells.
  • LINC00987 downregulation reduces ADR resistance in vitro and in vivo, while overexpression enhances it.
  • LINC00987 acts as a competing endogenous RNA for miR-4458, leading to HMGA2 upregulation and increased ADR resistance.

Conclusions:

  • LINC00987 downregulation mitigates ADR resistance by enabling miR-4458 to reduce HMGA2 levels.
  • LINC00987 emerges as a potential therapeutic target for overcoming chemoresistance in AML.
  • Targeting the LINC00987/miR-4458/HMGA2 axis could be a promising strategy for treating drug-resistant AML.
Keywords:
Acute myeloid leukemiaAdriamycinLong non-coding RNAmicroRNA

More Related Videos

Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype14:51

Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype

Published on: June 17, 2022

3.2K
Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells09:16

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Published on: September 1, 2019

7.5K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K