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Updated: Jun 23, 2025

A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines

A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines

Published on: January 14, 2017

Lymphoblastoid cell lines do not recapitulate physiological circulating B cell subtypes.

Connie MacKinnon¹, Ryan McLean¹, Antonia L Pritchard^1,2

¹Genetics and Immunology Department, Division of Medical Science, Institute of Health Research and Innovation, University of the Highlands and Islands, An Lochran, 10 Inverness Campus, IV2 5NA, UK.

Current Research in Immunology

|June 24, 2024

Summary

This summary is machine-generated.

Lymphoblastoid cell lines (LCLs) are often not physiologically representative of B cells due to Epstein-Barr virus (EBV) transformation. This study reveals that LCLs commonly display altered marker combinations, impacting their use in B cell research.

Keywords:

B cell subtypes EBV immortalised transformed B cells LCL Lymphoblastoid cells Markers

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The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

Published on: December 14, 2016

Area of Science:

Immunology
Cell Biology

Background:

Lymphoblastoid cell lines (LCLs), derived from Epstein-Barr virus (EBV) transformed B lymphocytes, are widely used in research.
The physiological relevance of LCLs for studying B cell function is debated due to inconsistent marker expression compared to primary B cells.

Purpose of the Study:

To evaluate the physiological representativeness of LCLs by applying a recently proposed consensus panel of B cell subtyping markers.
To characterize B cell subtypes within LCLs and identify alterations induced by EBV transformation.

Main Methods:

Application of a consensus panel of core B cell subtyping markers to LCLs.
Comparative analysis of marker expression in LCLs versus peripheral B cells.

Main Results:

Most LCLs exhibited marker combinations not found on physiological peripheral B cells.
Key B cell markers, including CD19 and CD21, were significantly altered during EBV-induced transformation to LCLs.
While most LCLs secreted IgG, the associated marker profiles were largely artifacts of in vitro EBV transformation.

Conclusions:

LCLs are generally not physiologically representative of their pre-transformed B cell subtypes.
Findings necessitate careful interpretation of past research using LCLs and inform the design of future studies.
The study highlights the profound impact of EBV transformation on B cell marker expression and function.