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Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools
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Mapping the distribution of differentiation potential for intestine, muscle, and hypodermis during early development

A E Cowan, J R McIntosh

    Cell
    |July 1, 1985
    PubMed

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    Determination01:51

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    During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...