Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
Liver Regeneration01:24

Liver Regeneration

3.2K
The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
3.2K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Linc01535 Promotes Hepatocellular Carcinoma Proliferation And Metastasis By Regulating The Mir-214-3p/vasp Axis

LINC01535 promotes hepatocellular carcinoma proliferation and metastasis by regulating the miR-214-3p/VASP axis

Chunjiang Liu1, Kuan Li2, Wenzhou Ding3

  • 1Department of General Surgery, Shaoxing People's Hospital, Shaoxing, 312000, China.

Journal of Cancer
|June 24, 2024

Related Experiment Videos

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
08:50

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

382
Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma
07:59

Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma

Published on: September 3, 2021

6.3K
CAM-Delam Assay to Score Metastatic Properties by Quantifying Delamination and Invasion Capacity of Cancer Cells
12:14

CAM-Delam Assay to Score Metastatic Properties by Quantifying Delamination and Invasion Capacity of Cancer Cells

Published on: June 2, 2022

2.6K

View abstract on PubMed

Summary
This summary is machine-generated.

Long noncoding RNA LINC01535 promotes hepatocellular carcinoma (HCC) progression by regulating the microRNA-214-3p (miR-214-3p)/VASP axis. This pathway activates PI3K/AKT signaling, driving tumor growth and metastasis in HCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • RNA Biology

Background:

  • Long noncoding RNAs (lncRNAs) are implicated in various carcinomas.
  • The specific role of LINC01535 in hepatocellular carcinoma (HCC) remains largely unexplored.

Purpose of the Study:

  • To investigate the function of LINC01535 in HCC proliferation and metastasis.
  • To elucidate the molecular mechanism underlying LINC01535's role in HCC, including its interactions with miR-214-3p and VASP.

Main Methods:

  • RNA sequencing (RNA-seq), cell counting kit-8 (CCK-8), colony formation, wound healing, Transwell, and tumor xenograft assays.
  • Fluorescence in situ hybridization (FISH), bioinformatics analysis, dual-luciferase assay, quantitative real-time polymerase chain reaction (qRT-PCR), and western blot analysis.
Keywords:
HCCLINC01535VASPmetastasis

Related Experiment Videos

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
08:50

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

382
Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma
07:59

Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma

Published on: September 3, 2021

6.3K
CAM-Delam Assay to Score Metastatic Properties by Quantifying Delamination and Invasion Capacity of Cancer Cells
12:14

CAM-Delam Assay to Score Metastatic Properties by Quantifying Delamination and Invasion Capacity of Cancer Cells

Published on: June 2, 2022

2.6K

Main Results:

  • LINC01535 is overexpressed in HCC tissues and cell lines, promoting HCC cell proliferation, migration, and invasion.
  • LINC01535 upregulation decreases miR-214-3p expression; miR-214-3p directly targets VASP.
  • LINC01535 sponges miR-214-3p, upregulating VASP, activating PI3K/AKT signaling, and promoting epithelial-to-mesenchymal transition (EMT).

Conclusions:

  • LINC01535 promotes HCC progression through the miR-214-3p/VASP axis and PI3K/AKT signaling pathway.
  • This study reveals a novel regulatory mechanism for LINC01535 in HCC.
  • LINC01535 presents a potential diagnostic and therapeutic target for HCC.
miR-214-3p
proliferation