JoVE - Journal of Visualized Experiments
JoVE Visualize
Contact Us

Coprisin/Compound K Conjugated Gold Nanoparticles Induced Cell Death through Apoptosis and Ferroptosis Pathway in Adenocarcinoma Gastric Cells

  • 0Graduate School of Biotechnology, and College of Life Science, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Republic of Korea.
Acs Omega +

|

June 24, 2024

|

June 24, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

This study reveals that gold nanoparticles combined with compound K and coprisin (GNP-CK-CopA3) effectively induce cancer cell death. The nanomedicine triggers both apoptosis and ferroptosis, offering a dual-targeting strategy against chemoresistant cancers.

Area Of Science

  • Biomedical Engineering
  • Nanotechnology
  • Cancer Biology

Background

  • Cancer cells can evade apoptosis through alternative cell death pathways like ferroptosis.
  • Targeting dual cell death pathways presents a promising strategy for diverse cancer types.
  • Previous work synthesized gold nanoparticles (GNP) with compound K (CK) and coprisin (CopA3) into GNP-CK-CopA3.

Purpose Of The Study

  • To assess the anticancer effects of GNP-CK-CopA3 on AGS cells.
  • To investigate the induction of apoptosis and ferroptosis by GNP-CK-CopA3.
  • To elucidate the underlying molecular mechanisms of GNP-CK-CopA3-induced cell death.

Main Methods

  • Cell viability assays and apoptosis induction studies using Hoechst, PI, and Annexin V-FITC/PI staining.
  • Mitochondrial disruption and cellular localization analysis via Mito-tracker and transmission electron microscopy.
  • Proteomic analysis, molecular docking, and dynamic simulations to identify molecular targets and mechanisms.

Main Results

  • GNP-CK-CopA3 demonstrated significant anticancer properties and induced apoptosis in AGS cells.
  • Proteomic analysis revealed altered protein expression, highlighting ferroptosis as a key pathway involving GPX4 and GSS.
  • Molecular simulations confirmed the binding of CopA3 and CK to GPX4 and GSS, supporting ferroptosis induction.

Conclusions

  • GNP-CK-CopA3 exhibits dual-action anticancer properties by inducing both apoptosis and ferroptosis.
  • This nanomedicine holds potential as a therapeutic strategy for chemoresistant cancers targeting multiple cell death pathways.