Fabrication of Mn-TPP/RGO Tailored Glassy Carbon Electrode for Doxorubicin Sensing

Area of Science:

• Electrochemistry
• Materials Science
• Nanotechnology

Background:

• Anticancer drug quantification is vital due to potential side effects.
• Electrochemical sensors offer a sensitive, affordable, and simple method for drug analysis.
• Doxorubicin (DOX) is a widely used anticancer drug requiring precise monitoring.

Purpose of the Study:

• To fabricate and characterize novel electrochemical sensors for doxorubicin detection.
• To evaluate the performance of modified glassy carbon electrodes (GCEs) for quantitative analysis of DOX.
• To assess the sensor's efficacy in real biological samples.

Main Methods:

• Synthesis and characterization of reduced graphene oxide (RGO), tetraphenylporphyrin decorated RGO (TPP/RGO), and manganese TPP/RGO nanostructures.
• Fabrication of GCEs modified with the synthesized nanomaterials.
• Electrochemical analysis using differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and cyclic voltammetry (CV).

Main Results:

• The Mn-TPP/RGO modified GCE demonstrated optimal performance at pH 3.
• The sensor exhibited a wide linear response range (0.1–0.6 mM), high sensitivity (112.09 μA mM⁻² cm⁻²), and a low detection limit (63.5 μM).
• DPV analysis yielded a limit of detection (LoD) of 27 μM and sensitivity of 0.174 μA μM⁻² cm⁻², with excellent stability, selectivity, and reproducibility. Reasonable recovery was observed in human serum analysis.

Conclusions:

• Manganese tetraphenylporphyrin decorated reduced graphene oxide modified GCE is a highly effective sensor for doxorubicin detection.
• The developed sensor shows great potential for sensitive and reliable quantification of doxorubicin in clinical applications.
• The sensor's performance in real samples highlights its practical applicability in therapeutic drug monitoring.