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Industrial Approach to Invertase Production from Fruit Waste for Enhanced Efficiency and Conservation
1Ege University, Department of Bioengineering, Faculty of Engineering, İzmir 35040, Turkey.
ACS Omega
|June 24, 2024
Summary
Fruit waste, like carob pulp, can be commercially repurposed for producing the invertase enzyme from Saccharomyces cerevisiae. Immobilization enhances enzyme stability and reusability, offering sustainable industrial applications.
Area of Science:
- Biotechnology
- Enzyme Technology
- Waste Valorization
Background:
- Fruit processing generates substantial waste, posing environmental and economic challenges.
- Enzymes like invertase have significant industrial applications, but their production can be costly.
- Repurposing agricultural byproducts offers a sustainable approach to enzyme sourcing.
Purpose of the Study:
- To investigate the commercial viability of using fruit waste for invertase enzyme production.
- To optimize the purification and immobilization of invertase from Saccharomyces cerevisiae.
- To evaluate the enhanced properties and reusability of the immobilized enzyme.
Main Methods:
- Culturing Saccharomyces cerevisiae using various fruit pulps (mulberry, carob, fig, grape) as nutrient sources.
- Enzyme extraction and purification involving ultrafiltration, precipitation, dialysis, and ion-exchange chromatography.
- Immobilization of purified invertase onto alginate beads.
- Characterization of enzyme activity, stability (pH, thermal), and performance in the presence of additives and solvents.
- Assessment of immobilized enzyme reusability over multiple cycles.
Main Results:
- Carob pulp medium yielded the highest invertase activity (12.90 U/mg protein), 2.5 times greater than peptone medium.
- Purification achieved a 12.11-fold increase with 26.93% yield.
- Immobilized invertase showed enhanced pH stability (optimal range 3.50-7.00) and thermal stability, especially at 65 °C.
- Activity increased by ~40% with Ca2+ and Mg2+ and was maintained in detergents and organic solvents.
- Immobilized enzyme retained 80% activity after 20 reuse cycles.
Conclusions:
- Fruit waste, particularly carob pulp, is a cost-effective substrate for invertase production.
- The developed purification and immobilization process yields a stable, reusable invertase enzyme.
- This approach offers a sustainable and economically viable method for enzyme production in the fruit juice industry, reducing waste and enhancing resource utilization.
