Related Experiment Video
Updated: Jun 23, 2025

Athymic Rat Model for Evaluation of Engineered Anterior Cruciate Ligament Grafts
Published on: March 26, 2015
The Influence of Different Hamstrings Assemblies on the Final Graft Diameter in Anterior Cruciate Ligament
Lúcio Flávio Biondi Pinheiro1, Marcos Henrique Frauendorf Cenni1, Thiago César Ladeira Estefani1
1Rede Mater Dei de Saúde, Belo Horizonte, MG, Brasil.
Abstract:
Objective This study aimed to compare gracilis and semitendinosus tendon graft diameters in anterior cruciate ligament (ACL) reconstruction using quadruple, quintuple, and sextuple assemblies. Another objective was to evaluate the percentage of patients in which each assembly type is possible, depending on the length of each free tendon. Methods Seventy-one patients underwent ACL reconstruction using hamstring tendons. We measured the diameters of the quadruple, quintuple, and sextuple assemblies in all patients. We recorded tendon length and graft diameter from three assembly types. Results Assembly comparison showed a statistically significant difference ( p < 0.001). In each assembly, graft diameter increased by 1 mm, a statistically significant value ( p < 0.001). In 2.8% of patients, the only potential assembly was the quadruple assembly because the free lengths of the 2 tendons removed were lower than 24 cm. The quintuple assembly was possible in 23.9% of subjects, as only the semitendinosus had a minimum length of 24 cm. The sextuple assembly was possible in 73.2% of patients because both tendons were at least 24 cm in length. Conclusion A quintuple or sextuple assembly is possible in 97.2% of cases since the final graft length of at least 8 cm is statistically significant between comparisons.
More Related Videos
08:30Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications
Published on: April 30, 2014
05:07Author Spotlight: Implementing the Enhanced Recovery After Surgery Concept in Rehabilitation Following Anterior Cruciate Ligament Reconstruction
Published on: March 1, 2024