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Updated: Jun 23, 2025
Ceramic Omnidirectional Bioprinting in Cell-Laden Suspensions for the Generation of Bone Analogs
Published on: August 8, 2022
Bifunctional mesoporous glasses for bone tissue engineering: Biological effects of doping with cerium and polyphenols
Ksenia Menshikh1, Ajay Kumar Reddy1, Andrea Cochis1
1Center for Translational Research on Autoimmune and Allergic Disease-CAAD, Department of Health Sciences, Università del Piemonte Orientale, Novara 28100, Italy.
Abstract:
This study evaluates the cytocompatibility of cerium-doped mesoporous bioactive glasses (Ce-MBGs) loaded with polyphenols (Ce-MBGs-Poly) for possible application in bone tissue engineering after tumour resection. We tested MBGs powders and pellets on 2D and 3D in vitro models using human bone marrow-derived mesenchymal stem cells (hMSCs), osteosarcoma cells (U2OS), and endothelial cells (EA.hy926). Promisingly, at a low concentration in culture medium, Poly-loaded MBGs powders containing 1.2 mol% of cerium inhibited U2OS metabolic activity, preserved hMSCs viability, and had no adverse effects on EA.hy926 migration. Moreover, the study discussed the possible interaction between cerium and Poly, influencing anti-cancer effects. In summary, this research provides insights into the complex interactions between Ce-MBGs, Poly, and various cell types in distinct 2D and 3D in vitro models, highlighting the potential of loaded Ce-MBGs for post-resection bone tissue engineering with a balance between pro-regenerative and anti-tumorigenic activities.
