JoVE - Journal of Visualized Experiments
JoVE Visualize
Contact Us

ESM1 May Be Used as a New Indicator for the Diagnosis and Prognosis of Early and Advanced Stage Digestive Tract Cancers

  • 0Department of Gastroenterology, Beijing Aerospace General Hospital, Beijing, People's Republic of China.
International Journal of General Medicine +

|

June 24, 2024

|

June 24, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Endothelial cell specific molecule 1 (ESM1) shows promise as a diagnostic and prognostic biomarker for stomach adenocarcinoma (STAD) and esophageal carcinoma (ESCA). Elevated ESM1 levels correlate with poor survival and impact cancer cell behavior.

Area Of Science

  • Oncology
  • Molecular Biology
  • Cancer Diagnostics

Background

  • Endothelial cell specific molecule 1 (ESM1) is a validated biomarker in various cancers.
  • Its role in stomach adenocarcinoma (STAD) and esophageal carcinoma (ESCA) requires further investigation.

Purpose Of The Study

  • To explore the expression patterns of ESM1 in STAD and ESCA.
  • To evaluate the diagnostic and prognostic value of ESM1 in these cancers.

Main Methods

  • Gene expression screening using the Gene Expression Omnibus database.
  • Quantitative analysis of ESM1 expression via reverse transcription-quantitative polymerase chain reaction and Western blot in patient samples.
  • Prognostic assessment using overall survival curves and diagnostic accuracy evaluation with receiver operating characteristic analysis.
  • In vitro assays to determine ESM1's effect on cell viability, migration, and invasion.

Main Results

  • High ESM1 expression correlated with poor overall survival in STAD and ESCA patients.
  • ESM1 levels were significantly elevated in both STAD and ESCA tissues.
  • ESM1 demonstrated high diagnostic accuracy (AUC > 0.79) for early and advanced stages.
  • ESM1 knockdown inhibited cell viability, migration, and invasion, while increasing apoptosis.

Conclusions

  • ESM1 serves as a potential novel biomarker for the diagnosis and prognosis of STAD and ESCA.
  • These findings support ESM1's utility in managing digestive tract cancers at various stages.

Keywords:

Related Concept Videos

Barrett Esophagus-I: Introduction 01:21

85

Barrett's esophagus is a medical condition where the esophageal mucosa is significantly damaged by stomach acid or other digestive fluids, often due to long-term exposure associated with gastroesophageal reflux disease (GERD). In GERD, a weakened or abnormally relaxed lower esophageal sphincter allows stomach acid to flow persistently into the esophagus.
This constant acid exposure transforms the esophagus's pink mucosal lining (stratified squamous epithelium) into a type of lining more...

Serum Laboratory Studies, Stool Test, Breath Test 01:30

333

Gastrointestinal (GI) diagnostic studies are pivotal in confirming, ruling out, diagnosing, or staging various diseases, including cancers. Following diagnosis, allocating time for discussions with the patient and providing informational resources is crucial. Diagnostic assessments of the GI tract often occur in outpatient settings like endoscopy suites or GI labs. Preparation for these tests may include dietary restrictions, fasting, liquid bowel preparations, laxatives, enemas, and the...

Barrett Esophagus-II: Clinical Manifestations and Management 01:21

136

Individuals with Barrett's esophagus are often asymptomatic, but they may experience symptoms commonly associated with GERD, such as heartburn and acid regurgitation. Additional symptoms can include difficulty swallowing, chest pain, unintentional weight loss, blood in the stool (which may appear black, tarry, or bloody), and episodes of vomiting.
To diagnose Barrett's esophagus, healthcare providers often recommend an endoscopy for those showing symptoms of acid reflux. The procedure...

Assessment of the Rectum and Anus 01:25

194

Evaluating the rectum and anus plays a crucial role in conducting a thorough physical examination of the gastrointestinal system. Although it may be uncomfortable and often embarrassing for the patient, it holds immense diagnostic value, particularly in detecting gastrointestinal diseases and abnormalities. This guide will explain how to perform this assessment using inspection and palpation methods.
Rectal Inspection
Begin by inspecting the perianal and anal areas for color, texture, rashes,...

Imaging Studies III: Gastrointestinal Motility Studies and Virtual Colonoscopy 01:26

74

This lesson explores three gastrointestinal imaging techniques: radionuclide testing, colonic transit studies, and virtual colonoscopy.
Radionuclide Testing
Radionuclide testing is a sophisticated medical technique for assessing gastrointestinal motility. It focuses on gastric emptying and colonic transit time. Radioactive markers track the movement of food through the digestive system, providing insights into gastrointestinal disorders.
In gastric emptying studies, a meal's liquid and...

Endoscopic Procedures I: Esophagogastroduodenoscopy 01:29

101

An Esophagogastroduodenoscopy (EGD) is a diagnostic procedure in which an endoscopist uses a flexible, lighted endoscope to visualize the upper gastrointestinal (GI) tract. The procedure includes visualizing the oropharynx, esophagus, stomach, and the first part of the small intestine, the duodenum.
During an EGD, the endoscope can be used to:

Diagnose conditions such as ulcers, GERD, celiac disease, and cancers.
Perform biopsies to collect small tissue samples for cancer or celiac disease...