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Updated: Jun 23, 2025
08:10
Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
Published on: August 8, 2016
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Current status and emerging frontiers in enzyme engineering: An industrial perspective
Obinna Giles Ndochinwa1, Qing-Yan Wang2, Oyetugo Chioma Amadi1
1Department of Microbiology, Faculty of Biological Science, University of Nigeria, Nsukka, Nigeria.
Heliyon
|June 24, 2024
Summary
Protein engineering enhances biocatalyst properties like stability and activity through methods such as mutagenesis and machine learning. This optimization is crucial for applications in biofuels and pharmaceuticals, improving enzyme function and sustainability.
Area of Science:
- Biochemistry and Organic Chemistry
- Enzyme Engineering
- Biotechnology
Background:
- Protein engineering offers efficient strategies to improve biocatalyst biochemical properties, including stability, activity, and stereoselectivity.
- Current limitations in predicting protein folding and function necessitate reprogramming existing enzymes via mutagenesis for desired traits.
- Naturally occurring enzymes often have limitations that protein engineering aims to overcome for industrial applications.
Purpose of the Study:
- To review recent trends in enzyme engineering for optimizing biocatalyst biochemical properties.
- To illustrate the significance of enzyme engineering through examples in biotechnology.
- To discuss the application of these methods for optimizing naturally occurring enzymes.
Main Methods:
- Protein engineering via mutagenesis to generate novel enzyme phenotypes.
- Enzyme immobilization and introduction of new chemical reactivities.
- Application of machine learning techniques for predicting protein structure, stability, and function.
Main Results:
- Engineered cellulases and hemicellulases exhibit enhanced robustness for biomass pretreatment in biofuel production.
- Tailored enzymes like cytochrome P450s and amine oxidases are developed for complex pharmaceutical synthesis.
- Machine learning aids in rational protein design, improving enzyme solubility, stability, and substrate specificity.
Conclusions:
- Enzyme engineering is essential for advancing biotechnology, offering tailored biocatalysts with improved properties.
- Optimized enzymes contribute to more economically viable and sustainable processes in biofuel and pharmaceutical industries.
- Continued research in enzyme engineering, including machine learning integration, promises further innovation in biocatalysis.
Keywords:
Directed evolutionEnzyme engineeringRationale designSite-directed mutagenesisSite-saturated mutagenesisMore Related Videos
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