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Related Experiment Video

Updated: Jun 23, 2025

Author Spotlight: Enhanced Urodynamic Method for Precise Urine Measurement in Awake Mice with Neurogenic Bladder
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Optimizing Mouse Urodynamic Techniques for Improved Accuracy.

Aytak Khabbaz1, Kristen Lynn Cohen1, Shengqi Zhang2

  • 1Department of Neurological Surgery, Indiana University School of Medicine; Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, Department of Neurological Surgery, Goodman and Campbell Brain and Spine, Indiana University School of Medicine.

Journal of Visualized Experiments : Jove
|June 24, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces a novel method using cyanoacrylate adhesive to prevent urine absorption during cystometry in awake mice. This technique improves the accuracy of voided volume measurements and stabilizes external urethral sphincter electromyography signals.

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Area of Science:

  • Urology
  • Neuroscience
  • Biomedical Engineering

Background:

  • Accurate measurement of lower urinary tract (LUT) parameters in awake mice is vital for studying conditions like neurogenic bladder after spinal cord injury (SCI).
  • Traditional cystometry in mice is challenged by urine absorption into fur and skin, leading to underestimated voided volumes (VV).
  • External urethral sphincter electromyography (EUS-EMG) recordings are also susceptible to artifacts in awake, moving animals.

Purpose of the Study:

  • To develop an improved method for accurate cystometry and EUS-EMG recordings in awake mice.
  • To overcome challenges of urine absorption and movement artifacts during urodynamic studies.

Main Methods:

  • A waterproof skin barrier was created around the urethral meatus and abdomen using cyanoacrylate adhesive.
  • This method aimed to prevent urine absorption and stabilize EUS electrodes.
  • Simultaneous cystometry and EUS-EMG recordings were performed in awake mice.

Main Results:

  • The cyanoacrylate barrier successfully prevented urine absorption, confirmed by consistent voided volume (VV) and residual volume (RV) measurements matching infused volumes.
  • No wetness was observed post-experiment, indicating effective urine containment.
  • Stable EUS-EMG signals were achieved with minimized artifacts from mouse movement and handling.

Conclusions:

  • The developed cyanoacrylate method significantly enhances the accuracy of urodynamic measurements in awake mice.
  • This technique is crucial for reliable preclinical research on LUT dysfunction and neurogenic bladder.
  • Improved methodologies are essential for advancing our understanding of urological conditions in animal models.