Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Identification of tubercle bacilli.

M D Yates, C H Collins

    Annales De Microbiologie
    |July 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Screening tubercle bacilli with 2-thiophene carboxylic acid hydrazide (TCH) and 2-furoic acid hydrazide (FAH) differentiates Mycobacterium tuberculosis strains. Further tests identify M. bovis, M. africanum, and BCG, aiding in tuberculosis diagnostics.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Preference Mapping of Fresh Tomatoes Across 3 Stages of Consumption.

    Journal of food science·2016
    Same author

    Toward cell-free biofuel production: Stable immobilization of oligomeric enzymes.

    Biotechnology progress·2014
    Same author

    Speciation of aqueous chromium(VI) solutions with the aid of Q-mode factor analysis followed by oblique projection.

    Talanta·2008
    Same author

    Determination of regional flavor differences in U.S. Cheddar cheeses aged for 6 mo or longer.

    Journal of food science·2008
    Same author

    Sensory properties of meal replacement bars and beverages made from whey and soy proteins.

    Journal of food science·2007
    Same author

    Chromosomal instability at a mutational hotspot in polyoma middle T-antigen affects its ability to activate the ARF-p53 tumor suppressor pathway.

    Oncogene·2005
    Same journal

    [Attempted characterization of microorganisms by their metallic profiles].

    Annales de microbiologie·1984
    Same journal

    Detection of aminopeptidases in Enterobacteriaceae by three simple chromogenic tests.

    Annales de microbiologie·1984
    Same journal

    Role of the virulence-associated plasmids of Yersinia enterocolitica on its immunogenicity against Y. pestis.

    Annales de microbiologie·1984
    Same journal

    Description of Corynebacterium tuberculostearicum sp. nov., a leprosy-derived Corynebacterium.

    Annales de microbiologie·1984
    Same journal

    [Antibiotic sensitivity of 139 strains of Listeria serotyped by the National Reference Center in 1983].

    Annales de microbiologie·1984
    Same journal

    Relationship between pathogenicity in footpad lesion test and invasiveness in Escherichia coli.

    Annales de microbiologie·1984
    See all related articles

    Area of Science:

    • Microbiology
    • Bacteriology
    • Infectious Diseases

    Background:

    • Accurate identification of mycobacterial species is crucial for effective tuberculosis diagnosis and treatment.
    • Traditional identification methods can be time-consuming and require specialized equipment.
    • Developing rapid and reliable screening methods is essential for clinical microbiology.

    Purpose of the Study:

    • To evaluate the utility of 2-thiophene carboxylic acid hydrazide (TCH) and 2-furoic acid hydrazide (FAH) for the initial screening of tubercle bacilli.
    • To establish a differential scheme for identifying Mycobacterium tuberculosis, M. bovis, and BCG strains.
    • To correlate phenotypic characteristics with phage typing groups in different geographical populations.

    Main Methods:

    • Initial screening of mycobacterial isolates using TCH and FAH.

    Related Experiment Videos

  • Subsequent differentiation using nitratase and oxygen requirement tests.
  • Identification of M. bovis and M. africanum through pyrazinamide sensitivity testing.
  • Correlation of results with phage typing groups (A, B, and I).

    • Main Results:

    • TCH and FAH resistance identified Mycobacterium tuberculosis strains.
    • Sensitive strains, including M. tuberculosis, M. bovis, and BCG, were further differentiated by nitratase and oxygen tests.
    • Pyrazinamide sensitivity distinguished M. bovis and M. africanum.
    • European M. tuberculosis strains were predominantly resistant to TCH/FAH (phage groups A/B), while Asian strains were sensitive (phage group I).

    Conclusions:

    • TCH and FAH serve as effective initial screening agents for differentiating tubercle bacilli.
    • A combination of biochemical tests provides a reliable method for identifying key mycobacterial species.
    • Geographical origin correlates with specific drug resistance patterns and phage types in M. tuberculosis.