Vitamin D-Regulated miR-589-3p in Patients with Cervical Cancer Predicts Patient Prognosis and is Involved in Tumor Progression
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View abstract on PubMed
Summary
This summary is machine-generated.Vitamin D/calcitriol can induce miR-589-3p, a microRNA that shows potential in predicting cervical cancer survival. This microRNA also inhibits cancer cell progression.
Area Of Science
- Oncology
- Molecular Biology
- Biochemistry
Background
- Cervical cancer prognosis remains a critical challenge.
- MicroRNAs (miRNAs) are increasingly recognized for their roles in cancer development and as potential biomarkers.
- Vitamin D and its active form, calcitriol, have demonstrated anti-cancer properties, but their molecular mechanisms, including miRNA regulation, require further elucidation.
Purpose Of The Study
- To investigate the role of Vitamin D/calcitriol-induced miR-589-3p in predicting cervical cancer patient prognosis.
- To evaluate the functional impact of miR-589-3p on cervical cancer cell behavior.
Main Methods
- Analysis of the GSE61829 dataset to identify differentially expressed miRNAs (DEMs) following calcitriol treatment.
- Verification of miR-589-3p expression in cervical cancer patients.
- Kaplan-Meier survival analysis and multivariate Cox proportional hazards model to assess the association between miR-589-3p and overall survival.
- MTT and Transwell assays to examine the effects of miR-589-3p on cervical cancer cell proliferation, migration, and invasion.
Main Results
- Eleven DEMs were identified from the GSE61829 dataset, including miR-589-3p.
- Cervical cancer tissues exhibited decreased miR-589-3p expression, which was upregulated after one year of Vitamin D intake.
- Low miR-589-3p levels after Vitamin D supplementation were a significant predictor of poor survival probability (p=0.0059) and increased death risk (HR: 3.04; 95% CI: 1.47-6.29; p=0.003).
- Overexpression of miR-589-3p suppressed the proliferation, migration, and invasion of both cervical cancer cells and calcitriol-treated cervical cancer cells.
Conclusions
- Vitamin D/calcitriol can induce miR-589-3p expression.
- miR-589-3p exhibits tumor-suppressive functions, inhibiting cervical cancer progression.
- miR-589-3p holds promise as a predictive biomarker for overall survival in cervical cancer patients.
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