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Updated: Jul 11, 2026

Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance
06:40

Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance

Published on: July 28, 2023

Prevalence of Helicobacter pylori resistance to certain antibiotics at An-Najah University Hospital: a

Qusay Abdoh1,2, Mohammad Alnees3,4,5, Lubna Kharraz6

  • 1Faculty of Medicine, An-Najah National University, Nablus, Palestine. Qusaygi@yahoo.com.

Scientific Reports
|June 24, 2024
PubMed

Insights

Antibiotic resistance in H. pylori is a major treatment challenge. This study in Palestine found high resistance to clarithromycin and metronidazole, but fluoroquinolones and amoxicillin remain effective options.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Pharmacology

Background:

  • Antibiotic resistance in Helicobacter pylori (H. pylori) significantly impacts treatment success rates.
  • Understanding local resistance patterns is crucial for effective H. pylori eradication strategies.

Purpose of the Study:

  • To determine the prevalence of antibiotic resistance in H. pylori isolates from dyspeptic patients in Palestine.
  • To evaluate the susceptibility of H. pylori to commonly used antibiotics.

Main Methods:

  • Gastric biopsies were collected from 91 dyspeptic patients undergoing esophagogastroduodenoscopy.
  • Microbiological assessment and antimicrobial susceptibility testing were performed on H. pylori isolates.
  • Resistance rates to Ciprofloxacin, Levofloxacin, Moxifloxacin, Amoxicillin, Metronidazole, and Clarithromycin were determined.

Main Results:

  • H. pylori was detected in 38.5% of the participants.
  • High resistance rates were observed for Metronidazole (100%) and Clarithromycin (47.4%).
  • Ciprofloxacin (0%), Levofloxacin (0%), Moxifloxacin (2.6%), and Amoxicillin (18.4%) showed higher efficacy.

Conclusions:

  • H. pylori strains in Palestine exhibit significant resistance to clarithromycin and metronidazole.
  • Fluoroquinolones and amoxicillin represent viable alternatives for H. pylori treatment in this region.
  • Judicious antibiotic use and consideration of quinolone-based regimens are recommended for H. pylori infections in Palestine.

Keywords:
Helicobacter pyloriAntibiotic resistanceAntimicrobial susceptibility testingCiprofloxacin

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