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Updated: Jun 23, 2025

Application of the Intelligent High-Throughput Antimicrobial Sensitivity Testing/Phage Screening System and Lar Index of Antimicrobial Resistance

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Published on: July 21, 2023

Lactobacilli decrease the susceptibility of Salmonella Typhimurium to azithromycin.

Lya Blais¹, Laurence Couture¹, Isabelle Laforest-Lapointe¹

¹Département de biologie, Université de Sherbrooke, Sherbrooke, Québec, Canada.

Microbiology Spectrum

|June 25, 2024

Summary

This summary is machine-generated.

Lactobacilli strains induce azithromycin tolerance in Salmonella Typhimurium through two distinct mechanisms: media acidification and metabolic changes. This highlights the importance of microbial interactions in antibiotic resistance.

Keywords:

Salmonella Typhimurium antibiotic tolerance azithromycin genetic screening lactobacilli pentamidine

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Area of Science:

Microbiology
Bacterial Interactions
Antibiotic Resistance

Background:

Bacteria engage in complex interactions during infections and within host-associated microbial communities.
Salmonella enterica serovar Typhimurium is a significant foodborne pathogen and a model organism for studying microbial community dynamics.

Purpose of the Study:

To investigate how lactobacilli strains influence the sensitivity of Salmonella Typhimurium to azithromycin.
To elucidate the mechanisms by which lactobacilli induce antibiotic tolerance in S. Typhimurium.

Main Methods:

Co-culturing S. Typhimurium with lactobacilli strains and exposing to acidified media.
Performing high-throughput single-gene deletion assays in S. Typhimurium under co-culture and acidic conditions.
Analyzing genes involved in envelope homeostasis, membrane permeability, and metabolic changes.

Main Results:

Lactobacilli co-culture and acidified media induced azithromycin tolerance in S. Typhimurium.
Acidification affected envelope homeostasis, while lactobacilli presence involved different mechanisms, potentially metabolic.
Both conditions identified genes related to envelope homeostasis and membrane permeability.

Conclusions:

Lactobacilli induce S. Typhimurium azithromycin tolerance via at least two distinct pathways.
Understanding these interactions is crucial for addressing antibiotic resistance in pathogens.
The broader microbial community context significantly influences bacterial responses to antibiotics.