COL10A1 expression distinguishes a subset of cancer-associated fibroblasts present in the stroma of high-risk basal cell carcinoma
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View abstract on PubMed
Summary
This summary is machine-generated.COL10A1 is identified as a key marker for high-risk basal cell carcinoma (BCC) subtypes, particularly sclerosing/infiltrative and basosquamous types. This finding offers potential for new prognostic biomarkers and targeted treatments for skin cancer.
Area Of Science
- Dermatology
- Oncology
- Molecular Biology
Background
- Basal cell carcinoma (BCC) is the most common human malignancy, with varying recurrence risks and aggressiveness among subtypes.
- The biological basis for BCC subtype differences is poorly understood, particularly the role of the tumor microenvironment and cancer-associated fibroblasts (CAFs).
Purpose Of The Study
- To investigate and characterize the stromal microenvironment of different basal cell carcinoma (BCC) subtypes.
- To focus on identifying specific cancer-associated fibroblast (CAF) populations and their role in BCC progression.
Main Methods
- Laser capture microdissection (LCM) followed by RNA sequencing (RNA-Seq) was employed to analyze stromal gene expression in 15 BCC samples across five pure subtypes.
- Healthy skin served as a control, and findings were validated using immunohistochemistry (IHC) and two independent single-cell RNA-Seq (scRNA-Seq) datasets.
Main Results
- Distinct gene expression signatures were observed in the stroma of different BCC subtypes, with nodular and micronodular being most similar, and superficial and sclerosing being most different.
- COL10A1 was found to be overexpressed in the stroma of high-risk sclerosing/infiltrative and basosquamous BCC subtypes, confirmed by IHC.
- scRNA-Seq analysis linked COL10A1 expression to CAFs in the stroma of infiltrative BCC, associated with extracellular matrix remodeling.
Conclusions
- COL10A1 serves as a significant marker for high-risk BCC subtypes, specifically sclerosing/infiltrative and basosquamous.
- A distinct CAF population expressing COL10A1 is associated with infiltrative BCC stroma, suggesting its role in tumor progression.
- COL10A1 presents potential as a novel prognostic biomarker for BCC, paving the way for tailored therapeutic strategies.
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