Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fibronectin in human trabecular drainage channels.

B B Floyd, P H Cleveland, D M Worthen

    Investigative Ophthalmology & Visual Science
    |June 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Inpatient treatment for depressed children and adolescents: preliminary evaluations.

    The Psychiatric hospital·1991
    Same author

    The role of interferons in the control of HIV replication in macrophages.

    Clinical immunology and immunopathology·1990
    Same author

    Interferons and bacterial lipopolysaccharide protect macrophages from productive infection by human immunodeficiency virus in vitro.

    The Journal of experimental medicine·1989
    Same author

    Glaucoma in blacks.

    Archives of ophthalmology (Chicago, Ill. : 1960)·1988
    Same author

    Enzyme immunofiltration staining assay for immediate diagnosis of herpes simplex virus and varicella-zoster virus directly from clinical specimens.

    Journal of clinical microbiology·1987
    Same author

    The affiliation partnership between U.S. medical schools and the Veterans Administration.

    The Alabama journal of medical sciences·1987
    Same journal

    Dual-Hit Myopia Mechanism Unveiled by Multi-Omics: Opn1mw Deficiency Primed the Retina for Exaggerated Response to Environmental Defocus.

    Investigative ophthalmology & visual science·2026
    Same journal

    Psychometric Performance of Children With Amblyopia During a Tablet-Based Adaptive Visual Acuity Assessment.

    Investigative ophthalmology & visual science·2026
    Same journal

    The Molecular Basis of Ocular Aging: Mechanisms, Pathologies, and Emerging Therapeutics.

    Investigative ophthalmology & visual science·2026
    Same journal

    Sensory Eye Dominance Remains Stable Across Binocular Combination and Rivalry in Normal and Anisometropic Amblyopic Vision.

    Investigative ophthalmology & visual science·2026
    Same journal

    Exosome-Associated Gene Network and the Role of SPP1 in Herpes Stromal Keratitis and the Therapeutic Modulation by Ursolic Acid.

    Investigative ophthalmology & visual science·2026
    Same journal

    Nonlinear Associations of Estimated Glucose Disposal Rate With Incident Age-Related Eye Diseases: Implications for Metabolic Risk Stratification.

    Investigative ophthalmology & visual science·2026
    See all related articles

    Fibronectin, an extracellular glycoprotein, is elevated in human aqueous drainage channels. This finding suggests its potential role in glaucoma pathophysiology.

    Area of Science:

    • Ophthalmology
    • Cell Biology
    • Biochemistry

    Background:

    • Fibronectin, an extracellular glycoprotein, is produced by human trabecular cells.
    • Previous studies suggest increased glycoproteins, including fibronectin, in glaucomatous eyes.
    • Fibronectin's role in the eye's aqueous drainage system requires further investigation.

    Purpose of the Study:

    • To develop a sensitive immunoassay for detecting fibronectin in human eye tissues.
    • To map the distribution patterns of fibronectin within human aqueous drainage channels.
    • To investigate the quantitative differences of fibronectin in drainage channels versus surrounding tissues.

    Main Methods:

    • Development of a sensitive immunoassay using avidin-biotinylated enzyme complex (ABC).

    Related Experiment Videos

  • Application of the immunoassay to frozen sections of human eyes.
  • Utilizing a perfusion technique to analyze fibronectin distribution in aqueous drainage channels.

    • Main Results:

    • The developed immunoassay successfully detected low levels of fibronectin.
    • Fibronectin was found to be present in human aqueous drainage channels.
    • Significantly larger quantities of fibronectin were observed in the aqueous drainage channels compared to surrounding tissues in older individuals.

    Conclusions:

    • Fibronectin is demonstrably present in human aqueous drainage channels.
    • The concentration of fibronectin is higher in these channels than in adjacent ocular tissues.
    • These findings contribute to understanding fibronectin's potential involvement in ocular physiology and pathology, possibly related to glaucoma.