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Updated: Jun 23, 2025
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Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
Published on: December 30, 2016
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Efficient Parahydrogen-Induced 13C Hyperpolarization on a Microfluidic Device.
Sylwia J Barker1,2, Laurynas Dagys1,3, Malcolm H Levitt1
1School of Chemistry, University of Southampton, Southampton SO17 1BJ, United Kingdom.
Journal of the American Chemical Society
|June 25, 2024
Summary
This study demonstrates direct 13C-hyperpolarized fumarate production using parahydrogen-induced polarization (PHIP) in a microfluidic lab-on-a-chip device. This breakthrough enhances metabolic quantification in cell cultures via microfluidic NMR.
Area of Science:
- Metabolic Engineering
- Biophysics
- Analytical Chemistry
Background:
- Microfluidic lab-on-a-chip (LoC) devices offer controlled environments for life science research.
- Hyperpolarized metabolites are crucial for quantifying metabolic processes using microfluidic NMR.
- Parahydrogen-induced polarization (PHIP) of 1H nuclei has been achieved in microfluidic systems, but longer spin lifetimes are needed for metabolic NMR.
Purpose of the Study:
- To demonstrate the direct production and detection of 13C-hyperpolarized fumarate using PHIP in a microfluidic LoC device.
- To achieve high 13C polarization for enhanced metabolic studies.
- To develop an efficient microfluidic PHIP method for producing hyperpolarized metabolites with long spin lifetimes.
Main Methods:
- Utilized a microfluidic lab-on-a-chip (LoC) device for parahydrogen-induced polarization (PHIP).
- Optimized the microfluidic PHIP device using finite element modeling.
- Achieved direct production and detection of 13C-hyperpolarized fumarate.
Main Results:
- Successfully produced and detected 13C-hyperpolarized fumarate with 8.5% polarization.
- This is the first demonstration of 13C-hyperpolarization of a metabolite by PHIP in a microfluidic device.
- The optimized device enables efficient production of 13C-hyperpolarized fumarate.
Conclusions:
- Direct 13C-hyperpolarization of fumarate in a microfluidic LoC device is feasible.
- This method significantly enhances the potential for in-situ metabolic quantification in cell cultures.
- The developed microfluidic PHIP technology provides a valuable tool for metabolic NMR studies.
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