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Related Concept Videos

¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

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The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
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¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

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When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
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Related Experiment Video

Updated: Jun 23, 2025

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
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Efficient Parahydrogen-Induced 13C Hyperpolarization on a Microfluidic Device.

Sylwia J Barker1,2, Laurynas Dagys1,3, Malcolm H Levitt1

  • 1School of Chemistry, University of Southampton, Southampton SO17 1BJ, United Kingdom.

Journal of the American Chemical Society
|June 25, 2024
PubMed
Summary

This study demonstrates direct 13C-hyperpolarized fumarate production using parahydrogen-induced polarization (PHIP) in a microfluidic lab-on-a-chip device. This breakthrough enhances metabolic quantification in cell cultures via microfluidic NMR.

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Area of Science:

  • Metabolic Engineering
  • Biophysics
  • Analytical Chemistry

Background:

  • Microfluidic lab-on-a-chip (LoC) devices offer controlled environments for life science research.
  • Hyperpolarized metabolites are crucial for quantifying metabolic processes using microfluidic NMR.
  • Parahydrogen-induced polarization (PHIP) of 1H nuclei has been achieved in microfluidic systems, but longer spin lifetimes are needed for metabolic NMR.

Purpose of the Study:

  • To demonstrate the direct production and detection of 13C-hyperpolarized fumarate using PHIP in a microfluidic LoC device.
  • To achieve high 13C polarization for enhanced metabolic studies.
  • To develop an efficient microfluidic PHIP method for producing hyperpolarized metabolites with long spin lifetimes.

Main Methods:

  • Utilized a microfluidic lab-on-a-chip (LoC) device for parahydrogen-induced polarization (PHIP).
  • Optimized the microfluidic PHIP device using finite element modeling.
  • Achieved direct production and detection of 13C-hyperpolarized fumarate.

Main Results:

  • Successfully produced and detected 13C-hyperpolarized fumarate with 8.5% polarization.
  • This is the first demonstration of 13C-hyperpolarization of a metabolite by PHIP in a microfluidic device.
  • The optimized device enables efficient production of 13C-hyperpolarized fumarate.

Conclusions:

  • Direct 13C-hyperpolarization of fumarate in a microfluidic LoC device is feasible.
  • This method significantly enhances the potential for in-situ metabolic quantification in cell cultures.
  • The developed microfluidic PHIP technology provides a valuable tool for metabolic NMR studies.