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Updated: Jun 23, 2025

Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins
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Palmitoylation promotes pores.

John F Foley1

  • 1Science Signaling, AAAS, Washington, DC 20005, USA.

Science Signaling
|June 25, 2024
PubMed
Summary
This summary is machine-generated.

Palmitoylation, a lipid modification, triggers pore formation in the plasma membrane. This occurs whether gasdermin D is intact or cleaved, impacting cell integrity.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Background:

  • Gasdermin D (GSDMD) is a key mediator of pyroptosis, a programmed cell death pathway.
  • GSDMD undergoes cleavage to release its N-terminal fragment, which is known to form pores.

Purpose of the Study:

  • To investigate the role of palmitoylation in gasdermin D-mediated pore formation.
  • To determine if palmitoylation affects both intact and cleaved gasdermin D.

Main Methods:

  • Biochemical assays to detect palmitoylation.
  • Cell-based assays to assess plasma membrane integrity.
  • Pore formation assays using purified gasdermin D.

Main Results:

  • Palmitoylation of gasdermin D was observed.
  • Palmitoylation of both intact and cleaved gasdermin D induced plasma membrane pore formation.
  • Lipid modification directly contributes to GSDMD's pore-forming activity.

Conclusions:

  • Palmitoylation is a critical regulatory mechanism for gasdermin D function.
  • This lipid modification can directly trigger plasma membrane permeabilization, independent of prior cleavage.
  • Findings reveal a novel aspect of pyroptosis regulation and GSDMD activity.