Related Experiment Video
Updated: Jun 23, 2025
Separation of Avian Preovulatory Follicle Granulosa and Theca Cell Layers for Downstream Applications
Published on: October 25, 2024
Hypothalamic transcriptome profile from laying period to incubation period of Changshun green-shell laying hens
1College of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun 558000, China; Qiannan Key Laboratory of Applied Biotechnology for Livestock and Poultry, Duyun 558000, China.
Abstract:
Incubation behavior in chickens is closely associated with hypothalamus. Here, RNA sequencing of hypothalamus from Changshun green-shell laying hens, an indigenous chicken breed from China, in egg-laying period (LP) and incubation period (BP) was conducted to identify critical pathways and candidate genes involved in controlling the incubation behavior in hypothalamus. A total of 637 up-regulated and 305 down-regulated differently expressed genes (DEGs) were identified in chicken hypothalamus between LP and BP groups. Gene ontology term (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis further revealed that neuroactive ligand-receptor interaction, hippo signaling pathway, and focal adhesion were significantly enriched. Five candidate genes (POMC, IGF1R, CHAD, VCL, and MYL9) were suggested to play crucial roles in the regulation of chicken incubation behavior. Our results further indicated the complexity of reproductive behavior of different chicken breeds.
