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Engineering circular RNA for molecular and metabolic reprogramming.

Narendra Kumar Sharma1, Pragya Dwivedi2, Ravi Bhushan3

  • 1Department of Bioscience and Biotechnology, Banasthali Vidyapith (Deemed University), P.O. Banasthali Vidyapith Distt. Tonk, Rajasthan, 304 022, India. drnarendraks@gmail.com.

Functional & Integrative Genomics
|June 25, 2024
PubMed
Summary
This summary is machine-generated.

Circular RNAs (circRNAs) offer a stable alternative to messenger RNA (mRNA) for prolonged protein expression. Engineering circRNAs enhances protein production for applications in molecular and metabolic reprogramming.

Keywords:
CRISPR/Cas-13CRISPR/Cas-9Circular RNAsGenetic engineeringIRESMetabolic reprogramming

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • RNA Engineering

Background:

  • Messenger RNA (mRNA) has versatile biological roles but is limited by short half-life and low translation efficiency.
  • Circular RNAs (circRNAs) are stable RNA molecules synthesized via back-splicing in eukaryotic cells.
  • Both synthetic and endogenous circRNAs can encode proteins, presenting them as a potential gene expression tool.

Purpose of the Study:

  • To summarize engineering strategies for exogenous circRNAs to extend protein expression duration.
  • To review systematic approaches for assembling circRNAs and optimizing protein production.
  • To explore how circRNA component optimization impacts translation for cellular reprogramming.

Main Methods:

  • Systematic review of engineering aspects for exogenous circRNA assembly.
  • Evaluation of factors influencing protein production from engineered circRNAs.
  • Analysis of optimization strategies for circRNA components (vector topology, UTRs, IRES, aptamers).

Main Results:

  • Engineering approaches can significantly prolong protein expression from circRNAs.
  • Optimization of circRNA components like vector topology and untranslated regions impacts protein output.
  • Engineered aptamers and internal ribosome entry sites (IRES) influence translation efficiency.

Conclusions:

  • Engineered circRNAs provide a robust platform for sustained protein expression.
  • CircRNA engineering enables precise control over protein production for molecular and metabolic reprogramming.
  • This technology offers novel therapeutic possibilities for regulating cellular features and treating diseases.