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Related Experiment Video

Updated: Jun 23, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

Sequencing-based analysis of microbiomes.

Yishay Pinto^1,2, Ami S Bhatt^3,4

¹Department of Genetics, Stanford University, Stanford, CA, USA.

Nature Reviews. Genetics

|June 25, 2024

Summary

This summary is machine-generated.

Recent technological advances are revolutionizing microbiome research. Scientists can now gain deeper insights into microbial community composition and function at unprecedented resolution.

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Last Updated: Jun 23, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Published on: May 2, 2018

Area of Science:

Microbiology
Genomics
Environmental Science

Background:

Microbiomes are diverse communities with significant roles in agriculture, environmental science, and human health.
Understanding microbiome function and composition is crucial across various scientific disciplines.

Purpose of the Study:

To highlight the impact of technological advancements on microbiome research.
To underscore the enhanced understanding of microbiome function and composition.

Main Methods:

Utilizing high-throughput DNA and RNA sequencing.
Employing improved long-read sequencing technologies.
Applying innovative computational analysis methods.

Main Results:

Deeper insights into microbiomes at genomic, transcriptional, and translational levels.
Understanding of microbiome function and composition beyond the species level.
Facilitation of microbiome research through technological and analytical progress.

Conclusions:

Technological and analytical advances have significantly improved microbiome studies.
Current methods allow for a more comprehensive understanding of microbiome roles and dynamics.
Future research can explore microbiome functions at higher resolutions.