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Updated: Jun 23, 2025

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Inhibition of Mir-21-5p Affects the Expression of LNCRNA X-Inactive Specific Transcript and Induces Apoptosis in

Samaneh Nejaddehghan1, Seyed Jalal Zargar1, Mana Oloomi2

  • 1Department of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran.

Iranian Journal of Public Health
|June 26, 2024
PubMed
Abstract

Insights

Inhibiting miR-21-5p in breast cancer cells increases lncRNA-XIST and apoptosis genes, promoting cell death. This suggests miR-21-5p inhibition is a potential therapeutic strategy for breast cancer.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Cell Biology

Background:

  • MicroRNA-21-5p (miR-21-5p) is implicated in various cancers.
  • Long non-coding RNA XIST (lncRNA-XIST) role in cancer is under investigation.
  • Understanding their interaction is crucial for breast cancer (BC) therapy.

Purpose of the Study:

  • To investigate the effect of miR-21-5p inhibition on lncRNA-XIST expression.
  • To determine the impact on apoptosis in MCF-7 breast cancer cells.
  • To explore potential therapeutic targets for BC.

Main Methods:

  • MCF-7 cells were transfected with anti-miR-21-5p oligonucleotide.
  • Gene expression analyzed by RT-qPCR (miR-21-5p, lncRNA-XIST, BAX, P53, BC200).
  • Cell viability, apoptosis, and cell cycle assessed using MTT and flow cytometry.

Main Results:

  • miR-21-5p and lncRNA-XIST expression were significantly down- and up-regulated, respectively.
  • Apoptosis-associated genes BAX and P53 showed significant upregulation.
  • Inhibition of miR-21-5p decreased cell viability and increased apoptosis.

Conclusions:

  • Inhibiting miR-21-5p upregulates lncRNA-XIST and apoptosis markers (BAX, P53) in MCF-7 cells.
  • This leads to induced apoptosis, suggesting a therapeutic potential for BC.
  • Further research may establish miR-21-5p inhibition as a target for BC molecular therapies.
Keywords:
ApoptosisBreast cancerNon-coding RNAX-inactive specific transcript

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