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CircABCB10 Promotes the Apoptosis and Inflammatory Response of 16HBE Cells by Cigarette Smoke Extract by Targeting

Changping Yun1, Yuguang Wang2, Dongxu Wang2

  • 1Department of Respiration, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar 161000, China.

Iranian Journal of Public Health
|June 26, 2024
PubMed
Abstract

Insights

Circular RNA ABCB10 (circABCB10) promotes chronic obstructive pulmonary disease (COPD) by targeting the miR-130a/PTEN axis, driving inflammation and apoptosis. This finding offers a potential therapeutic target for COPD treatment.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Pulmonology

Background:

  • Chronic obstructive pulmonary disease (COPD) presents a significant global health challenge due to high mortality rates.
  • Effective therapeutic strategies for COPD remain urgently needed.

Purpose of the Study:

  • To investigate the role of circABCB10 in the pathogenesis of COPD.
  • To elucidate the molecular mechanism involving circABCB10, miR-130a, and PTEN in COPD development.

Main Methods:

  • Predicted the circABCB10, miR-130a, and PTEN targeting relationship using the TargetScan database.
  • Analyzed circABCB10, miR-130a, and PTEN expression in lung tissues of COPD patients and CSE-treated 16HBE cells via qRT-PCR.
  • Assessed inflammatory factors, apoptotic proteins, and cell proliferation using ELISA, Western Blot, and CCK8 assays, respectively.

Main Results:

  • CircABCB10 expression was elevated in COPD lung tissues and CSE-exposed 16HBE cells.
  • Knockdown of circABCB10 reduced CSE-induced inflammation, an effect reversed by miR-130a inhibition.
  • Overexpression of PTEN counteracted the effects of circABCB10 knockdown, modulating inflammation and apoptosis.

Conclusions:

  • CircABCB10 exacerbates inflammatory responses in COPD by targeting the miR-130a/PTEN axis.
  • The circABCB10/miR-130a/PTEN pathway represents a potential therapeutic target for managing COPD.
Keywords:
ApoptosisCellsChronic obstructive pulmonary diseaseImmunology

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