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Updated: Jun 23, 2025

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
The complete mitochondrial genome of a Biwa goby, Gymnogobius isaza (Tanaka, 1916)
Nao Nagao1, Saki Hiraoka1, Tsuyoshi Mori1
1Faculty of Bio-Science, Nagahama Institute of Bio-Science and Technology, Shiga, Japan.
Abstract:
We determined the complete mitochondrial DNA sequence of a Biwa goby, Gymnogobius isaza (Tanaka, 1916) using next-generation sequencing methods. The composition of its mitogenome is the same as that observed in most other vertebrates, comprising of 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and two control regions. Our molecular phylogenetic analysis confirmed the close phylogenetic relationship between G. isaza and G. petschiliensis. This mitogenome information will be useful for distribution surveys using environmental DNA and the development of conservation strategies for this species.

