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lncRNA - Long Non-coding RNAs02:39

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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Cardiac Development Long Non-Coding RNA (CARDEL) Is Activated during Human Heart Development and Contributes to

Isabela T Pereira1, Rubens Gomes-Júnior1, Aruana Hansel-Frose1

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Researchers identified CARDEL, a long non-coding RNA, crucial for heart development. Its expression is vital for cardiac lineage commitment and cardiomyocyte function in vitro.

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cardiac developmentcardiomyocytesgene expressionlncRNA

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Area of Science:

  • Cardiovascular Biology
  • Stem Cell Biology
  • Genetics

Background:

  • Human heart development relies on complex gene networks.
  • Human pluripotent stem cells (hPSCs) are valuable tools for studying cardiac differentiation.
  • Understanding cardiac gene regulation is key to regenerative medicine.

Purpose of the Study:

  • To identify novel genes involved in cardiac development.
  • To investigate the role of CARDEL in cardiac cell-fate commitment.
  • To elucidate the function of CARDEL in hPSC-derived cardiomyocytes.

Main Methods:

  • In vitro cardiac differentiation of hPSCs.
  • Gene expression analysis in fetal and adult heart tissues.
  • CRISPR-Cas9 gene editing for CARDEL knockout.
  • Overexpression studies of CARDEL in hPSCs.

Main Results:

  • Identified CARDEL (CARdiac DEvelopment Long non-coding RNA) expression correlating with cardiac lineage commitment.
  • CARDEL knockout hPSCs exhibited impaired cardiac differentiation.
  • Overexpression of CARDEL enhanced beating rates in hPSC-derived cardiomyocytes.

Conclusions:

  • CARDEL plays a significant role in cardiac development and cell-fate commitment.
  • CARDEL is a key regulator of cardiomyocyte formation and function.
  • This study provides molecular evidence for CARDEL's contribution to the cardiac program.