Progress in Core-Shell Magnetic Mesoporous Materials for Enriching Post-Translationally Modified Peptides

Area of Science:

• Biomarker Discovery
• Analytical Chemistry
• Materials Science

Background:

• Endogenous peptides with post-translational modifications are crucial disease biomarkers.
• Challenges include low abundance, weak ionization, and interference in biological samples, necessitating advanced enrichment techniques.
• Existing methods like mesoporous materials face limitations such as peptide loss during separation.

Purpose of the Study:

• To review recent advancements in core-shell magnetic mesoporous materials for peptide enrichment.
• To evaluate the performance of these materials in isolating glycopeptides and phosphopeptides.
• To compare different functionalization strategies for enhanced peptide capture.

Main Methods:

• Utilizing core-shell magnetic mesoporous materials for selective peptide separation and enrichment.
• Employing mass spectrometry for the analysis of enriched peptides.
• Comparing enrichment efficiency across various functionalized material types.

Main Results:

• Core-shell magnetic mesoporous materials demonstrate high biocompatibility, efficient enrichment, and excellent recoverability.
• These materials effectively isolate target glycopeptides and phosphopeptides from complex biological matrices.
• Different functionalization methods exhibit varying impacts on enrichment performance.

Conclusions:

• Core-shell magnetic mesoporous materials represent a promising platform for sensitive biomarker analysis.
• Optimized functionalization is key to maximizing the potential of these materials for glycopeptide and phosphopeptide enrichment.
• This review highlights the progress and future directions in using these materials for disease diagnostics.