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Related Concept Videos

  1. Home
  3. Research Domains
  5. Agricultural, Veterinary And Food Sciences
  7. Crop And Pasture Production
  9. Crop And Pasture Improvement (incl. Selection And Breeding)
  11. Boosting Prrsv-specific Cellular Immunity: The Immunological Profiling Of An Fc-fused Multi-ctl Epitope Vaccine In Mice.
  1. Home
  3. Research Domains
  5. Agricultural, Veterinary And Food Sciences
  7. Crop And Pasture Production
  9. Crop And Pasture Improvement (incl. Selection And Breeding)
  11. Boosting Prrsv-specific Cellular Immunity: The Immunological Profiling Of An Fc-fused Multi-ctl Epitope Vaccine In Mice.

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Boosting PRRSV-Specific Cellular Immunity: The Immunological Profiling of an Fc-Fused Multi-CTL Epitope Vaccine in Mice.

Xinnuo Lei1, Jinzhao Ban1,2, Zhi Wu1

  • 1Jiangsu Key Laboratory for High-Tech Research and Development of Veterinary Biopharmaceuticals, Engineering Technology Research Center for Modern Animal Science and Novel Veterinary Pharmaceutic Development, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China.

Veterinary Sciences
|June 26, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

A novel recombinant protein, pFc-PTE, shows promise as a safe and effective vaccine candidate against Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). It stimulates a robust and long-lasting cellular immune response, offering a potential breakthrough for swine farming.

Keywords:
CTL epitopeFccellular immunityporcine reproductive and respiratory syndrome virus (PRRSV)

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Area of Science:

  • Veterinary Immunology
  • Vaccine Development
  • Molecular Biology

Background:

  • Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) poses a significant global threat to swine health and the agricultural economy.
  • Conventional PRRSV vaccines exhibit limitations in protection efficacy and present biosecurity concerns.

Purpose of the Study:

  • To develop a novel vaccine candidate targeting PRRSV by creating a multi-epitope peptide fused with a porcine Fc fragment.
  • To evaluate the immunogenicity, safety, and duration of the immune response induced by the recombinant protein pFc-PTE.

Main Methods:

  • Identification of 10 PRRSV-specific cytotoxic T lymphocyte (CTL) epitopes using enzyme-linked immunospot assay (ELISPOT).
  • Construction of a multi-epitope peptide (PTE) and its fusion with a modified porcine Fc molecule to generate pFc-PTE.
vaccine
  • Assessment of cellular immune response, including interferon-gamma (IFN-γ) secretion and Th1 response, in immunized mice.

    • Main Results:

    • The recombinant protein pFc-PTE effectively stimulated PRRSV-specific splenic lymphocytes to produce high levels of IFN-γ.
    • pFc-PTE demonstrated a comparable cellular immune response to PTE alone but with a significantly extended duration of at least 10 weeks.
    • The vaccine candidate was predicted to be non-toxic and non-allergenic, predominantly inducing a Th1-biased immune response.

    Conclusions:

    • pFc-PTE represents a promising novel vaccine candidate for PRRSV, offering enhanced safety and potency.
    • The findings suggest pFc-PTE's potential to improve cellular immunity and provide a new strategy for viral vaccine design.