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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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Related Experiment Video

Updated: Jun 23, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

Digitized Kinetic Analysis Enhances Genotyping Capacity of CRISPR-Based Biosensing.

Joon Soo Park¹, Patarajarin Akarapipad¹, Fan-En Chen¹

¹Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21218, United States.

|June 26, 2024

Summary

This summary is machine-generated.

We developed SMART-dCRISPR, a novel CRISPR/Cas12a biosensing method. It uses single-molecule kinetics to accurately genotype SARS-CoV-2 mutations, improving nucleic acid detection.

Keywords:

CRISPR SARS-CoV-2 digital genotyping kinetic analysis

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Last Updated: Jun 23, 2025

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Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

Area of Science:

Molecular Biology
Biosensing Technology
Genomics

Background:

CRISPR/Cas systems offer sequence-specific nucleic acid detection.
Existing CRISPR genotyping methods struggle with distinguishing similar sequences due to Cas effector mismatch tolerance.
Comprehensive crRNA screening is often required for accurate genotyping.

Purpose of the Study:

To introduce a novel CRISPR-based genotyping method, SMART-dCRISPR, overcoming limitations of existing techniques.
To leverage single-molecule kinetic analysis for enhanced mutation detection.
To apply the method for genotyping SARS-CoV-2 variants.

Main Methods:

Developed Single-Molecule kinetic Analysis via Real-Time digital CRISPR/Cas12a-assisted assay (SMART-dCRISPR).
Utilized differential signal kinetics based on crRNA-target complementarity for mutation discernment.
Employed single-molecule digital measurements to analyze kinetic profiles.
Applied the assay to genotype SARS-CoV-2 mutations (K417N, 69/70DEL) in clinical samples.

Main Results:

SMART-dCRISPR successfully distinguished wild-type, Omicron BA.1, and Omicron BA.2 SARS-CoV-2 strains.
The method accurately genotyped specific point mutations and deletions in clinical nasopharyngeal/nasal swab samples.
Differential kinetic profiles enabled mutation detection obscured by target concentration variations.

Conclusions:

SMART-dCRISPR enhances CRISPR-based genotyping by analyzing single-molecule kinetics.
The assay accurately identifies SARS-CoV-2 variants and mutations.
A portable digital device integration makes SMART-dCRISPR practical for point-of-care settings.