Related Experiment Video
Updated: Jun 23, 2025
08:58
Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
6.4K
Comparative resistomics analysis of multidrug-resistant Chryseobacteria
1Department of Chemistry and Environmental Science, New Jersey Institute of Technology, Newark, New Jersey, USA.
Environmental Microbiology Reports
|June 26, 2024
Summary
Multidrug-resistant Chryseobacteria found in wastewater harbor numerous antibiotic resistance genes (ARGs). A new resistance cluster and mobile genetic elements suggest potential for spread, complicating infection control.
Area of Science:
- Environmental microbiology
- Genomics
- Antimicrobial resistance
Background:
- Chryseobacteria are significant human pathogens causing nosocomial infections.
- Multidrug resistance in bacteria poses a growing global health threat.
Purpose of the Study:
- To investigate the genomic basis of antibiotic resistance in Chryseobacteria isolated from wastewater.
- To compare the resistome of environmental Chryseobacteria with clinical isolates.
Main Methods:
- Genome sequencing of four multidrug-resistant Chryseobacteria using Nanopore technology.
- Comparative resistomics analysis with 211 public Chryseobacteria genomes.
- Minimum inhibitory concentration (MIC) testing for antibiotic resistance.
Main Results:
- A majority of Chryseobacteria possess multiple antibiotic resistance genes (ARGs), conferring resistance to several antimicrobial classes.
- A novel resistance gene cluster (catB11, tetX, MGEs) was identified, with genes enriched in clinical isolates.
- ARGs co-located with mobile genetic elements (MGEs) indicate potential for horizontal gene transfer.
Conclusions:
- While many ARGs in Chryseobacteria appear intrinsic, the identification of a new resistance cluster and ARG-MGE co-localization highlights mechanisms for resistance spread.
- Interspecies and intergenera transfer of ARGs may accelerate dissemination in clinical settings, challenging infection control strategies.
