Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

355
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
355
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

324
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
324
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

4.2K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
4.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Long ranged stress correlations in the hard sphere liquid.

The Journal of chemical physics·2024
Same author

Temporal Evolution of Interparticle Potentials of PMMA Colloids in CHB/Decalin.

Langmuir : the ACS journal of surfaces and colloids·2024
Same author

Decoupling of rotation and translation at the colloidal glass transition.

The Journal of chemical physics·2024
Same author

Observation of liquid glass in molecular dynamics simulations.

The Journal of chemical physics·2024
Same author

Electronically Preresonant Stimulated Raman Scattering Microscopy of Weakly Fluorescing Chromophores.

The journal of physical chemistry. B·2023
Same author

Influence of chain length and branching on poly(ADP-ribose)-protein interactions.

Nucleic acids research·2023
Same journal

Spectroscopic Investigation of the In Vivo Light-Dependent Photodynamics of the Marine Diatom Phaeodactylum tricornutum.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same journal

Atomistic Insights into the Thermal Decomposition and Runaway Mechanism of Peroxypropionic Acid.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same journal

Hydrazine Adsorption on Hexagonal Ice (0001): First-Principles Investigations on Stability, Dynamics, and Chirality Changes.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same journal

Sustainable Ball Milling-Assisted Synthesis of Bread Waste-Derived Highly Porous Carbons for Adsorption-Based Applications.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same journal

RNALig: An ML-Driven Structure-Based Scoring Function for Estimating Binding Affinities of RNA-Ligand Complexes.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same journal

Photoswitchable Polar Azobenzene-Based Liquid Crystals for Electro-Optic and Optical Data Storage Applications.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 23, 2025

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

3.9K

High Sensitivity Stimulated Raman Scattering Microscopy with Electronic Resonance Enhancement.

Andrea Pruccoli1, Andreas Zumbusch1

  • 1Department of Chemistry, University of Konstanz, Universitätsstraße 10, 78464, Konstanz, Germany.

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
|June 26, 2024
PubMed
Summary
This summary is machine-generated.

This review enhances stimulated Raman scattering (SRS) microscopy sensitivity to micromolar levels using pre-resonant excitation. This breakthrough overcomes limitations in label-free imaging, enabling new scientific investigations.

Keywords:
Raman microscopyRaman scatteringbioimagingnon-linear microscopynon-linear spectroscopy

More Related Videos

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

3.9K
Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

7.6K

Related Experiment Videos

Last Updated: Jun 23, 2025

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

3.9K
Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

3.9K
Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

7.6K

Area of Science:

  • Optical microscopy
  • Spectroscopy
  • Biomedical imaging

Background:

  • Spontaneous Raman microscopy is label-free but slow and prone to fluorescence.
  • Coherent Raman microscopy (CARS, SRS) is fast and fluorescence-resistant but lacks sensitivity.
  • Low sensitivity limits the broader application of current coherent Raman techniques.

Purpose of the Study:

  • To review sensitivity enhancement strategies for stimulated Raman scattering (SRS) microscopy.
  • To discuss the use of electronically pre-resonant excitation for improving SRS sensitivity.
  • To explore new experimental developments and applications enabled by enhanced SRS microscopy.

Main Methods:

  • Utilizing electronically pre-resonant excitation to boost SRS signal.
  • Discussing the technological implementation of enhanced SRS microscopy.
  • Presenting successful applications and new experimental developments.

Main Results:

  • Achieved SRS microscopy sensitivity down to micromolar (µM) detection levels.
  • Demonstrated the effectiveness of electronically pre-resonant excitation for sensitivity enhancement.
  • Showcased successful applications and novel experimental capabilities.

Conclusions:

  • Electronically pre-resonant excitation significantly enhances SRS microscopy sensitivity.
  • Enhanced SRS microscopy overcomes previous sensitivity limitations for broader applications.
  • New experimental developments open avenues for advanced label-free investigations.