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Related Experiment Video

Updated: May 11, 2026

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

Published on: October 4, 2012

Single-cell proteomics differentiates Arabidopsis root cell types.

Christian Montes¹, Jingyuan Zhang², Trevor M Nolan^2,3

¹Department of Plant Pathology, Entomology, and Microbiology, Iowa State University, Ames, IA, 50011, USA.

The New Phytologist

|June 26, 2024

Summary

This summary is machine-generated.

Single-cell proteomics (SCP) successfully analyzed plant root cells, differentiating adjacent cell types like cortex and endodermis. This approach identifies cell-specific proteins, advancing functional genomics in plants.

Keywords:

Arabidopsis cell type cortex endodermis proteomics root single cell

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Last Updated: May 11, 2026

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Isolation and Transcriptome Analysis of Plant Cell Types

Isolation and Transcriptome Analysis of Plant Cell Types

Published on: April 7, 2023

Area of Science:

Plant biology
Proteomics
Cellular heterogeneity

Background:

Single-cell proteomics (SCP) is a powerful technique for studying cellular differences.
Understanding cellular heterogeneity is crucial in multicellular organisms.
Applying SCP to plants is a developing area.

Purpose of the Study:

To demonstrate the feasibility of single-cell proteomics in plant samples.
To analyze cellular heterogeneity in adjacent plant root cell types.
To identify cell-specific protein markers for differentiation.

Main Methods:

Utilized single-cell proteomics on isolated root cells from Arabidopsis thaliana.
Focused on cortex and endodermis cell types.
Analyzed protein quantification and expression enrichment.

Main Results:

Successfully applied SCP to 756 plant root cells.
Identified 3217 proteins after stringent filtering.
Discovered 596 proteins enriched in either cortex or endodermis cells, enabling differentiation.

Conclusions:

Single-cell proteomics can resolve distinct, neighboring plant cell types.
SCP facilitates the discovery of biomarkers for functional genomics.
This study validates SCP for plant research and cell-type specific analysis.