Design and Probing of Prism-Based SPR Nano-Biosensor for Human Sperm Detection

Area of Science:

• Biomedical Engineering
• Materials Science
• Reproductive Biology

Background:

• Human sperm function is vital for natural reproduction, but environmental factors can impair it.
• Current computer-aided detection methods lack precision, especially for low sperm concentrations.
• Accurate sperm analysis is essential for diagnosing infertility and monitoring reproductive health.

Purpose of the Study:

• To develop and demonstrate a novel sulfide material-based nano-biosensor for enhanced human sperm sample detection.
• To improve the precision and sensitivity of sperm analysis, particularly for samples with low sperm concentrations.
• To utilize surface plasmon resonance (SPR) principles for a highly accurate detection system.

Main Methods:

• A prism-based Kretschmann configuration SPR nano-biosensor was designed and fabricated.
• The sensor structure incorporates silver, silicon, a sulfide layer, and black phosphorus.
• Seven human sperm samples were analyzed, measuring concentration, mobility, and refractive index using a refractometer.

Main Results:

• The proposed sulfide material-based SPR nano-biosensor achieved a maximum sensitivity of [insert value].
• The sensor demonstrated a high Q-factor (QF) of [insert value] and a detection accuracy (DA) of 1.37.
• These results represent a substantial improvement over existing methods for sperm detection.

Conclusions:

• The developed sulfide material-based SPR nano-biosensor shows significant potential for precise and sensitive human sperm analysis.
• This technology can overcome the limitations of current methods, especially for samples with low sperm concentrations.
• The findings contribute to advancing diagnostic tools for reproductive health and fertility assessments.