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Related Experiment Video

Updated: Jun 23, 2025

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Simple Characterization of Cylindrical Diffuser Fibers With a Fluorescent Layer.

Herbert Stepp¹, Ronald Sroka¹

¹Laser-Forschungslabor, LIFE Center, University Hospital, LMU Munich, Planegg, Germany.

Lasers in Surgery and Medicine

|June 26, 2024

Summary

A new method accurately measures light diffuser profiles using fluorescence, eliminating angle dependence. This technique is valuable for quality assurance of diffusers used in laser therapies.

Keywords:

cylindrical diffuser fiber intensity profile interstitial laser thermotherapy photodynamic therapy

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Last Updated: Jun 23, 2025

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Published on: October 9, 2014

Area of Science:

Optics and Photonics
Biomedical Engineering
Medical Imaging

Background:

Cylindrical diffusers (CDFs) are crucial in laser-based medical therapies.
Accurate measurement of light emission profiles is essential for CDF quality assurance.
Existing methods may suffer from angle dependency and environmental factors.

Purpose of the Study:

To develop a fast, simple, versatile, and reliable method for recording light emission intensity profiles of CDFs.
To create an angle-independent measurement technique.
To assess the impact of different media on emission profiles.

Main Methods:

Utilized a fluorescent color glass filter (RG695) to convert red light (635 nm diode laser) into near-infrared fluorescence.
Imaged the near-infrared fluorescence using a camera.
Processed camera images to obtain emission intensity profiles.
Compared CDF profiles from four manufacturers in air and transparent liquids.

Main Results:

The developed method provides angle-independent intensity profiles with a single camera shot.
Emission profiles significantly change in media with different refractive indices, depending on the diffuser tip's emission principle.
Demonstrated the method's versatility in air and transparent liquids.

Conclusions:

Converting emitted light to fluorescence eliminates angle dependence in recorded profiles.
The method facilitates easy assessment of refractive index (mis)matching by immersing the setup in liquids.
This measurement principle has potential for quality assurance of CDFs in interstitial laser thermotherapy and photodynamic therapy.