Green method by National Environmental Methods Index and Eco-Scale Assessment for evaluation of gatifloxacin-based product by HPLC
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.A new green analytical method quantifies gatifloxacin (GFX) in eye drops using HPLC. Evaluated by NEMI and ESA, this validated method offers an environmentally friendly approach for pharmaceutical analysis.
Area Of Science
- Analytical Chemistry
- Green Chemistry
- Pharmaceutical Analysis
Background
- Gaps exist in green analytical methods for gatifloxacin (GFX) products.
- National Environmental Methods Index (NEMI) and Eco-Scale Assessment (ESA) aid in evaluating analytical method environmental impact.
Purpose Of The Study
- Develop and validate a green high-performance liquid chromatography (HPLC) method for quantifying GFX in eye drops.
- Assess the method's environmental friendliness using NEMI and ESA.
Main Methods
- HPLC using a C8 column, purified water/acetic acid/ethanol mobile phase, and UV detection at 292 nm.
- Method validation included linearity, precision, selectivity, accuracy, and robustness testing.
- Greenness assessed via NEMI scoring and ESA evaluation.
Main Results
- The method showed excellent linearity (2-20 μg/mL) and precision (RSD < 3.45%).
- It demonstrated selectivity, accuracy (101.07% recovery), and robustness.
- NEMI indicated four green quadrants, and ESA scored the method at 88, signifying high environmental friendliness.
Conclusions
- A validated, green HPLC method for GFX quantification in eye drops was successfully developed.
- The method meets green chemistry principles, supported by NEMI and ESA assessments.
- This provides a sustainable analytical solution for pharmaceutical quality control.
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.