Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From Sea to Cell: <i>Ascophyllum nodosum</i> and <i>Fucus vesiculosus</i> Extracts Attenuate NF-κB-Mediated Inflammation and Protect Intestinal Barrier Integrity-A Comprehensive Analysis Applying In Vitro and In Vivo Models.

Marine drugs·2026
Same author

An exploratory study to investigate the effects of motor performance and maturation on talent selection in handball.

Frontiers in psychology·2026
Same author

Phospholipid-driven conformational switching of HCV NS5A links protein folding to replication membrane remodeling.

Science advances·2026
Same author

Single-molecule dynamics reveal ATP binding alone powers substrate translocation by an ABC transporter.

Nature communications·2026
Same author

Braess' Paradox in Enzyme Kinetics: Asymmetry from Population Balance without Direct Cooperativity.

Journal of chemical theory and computation·2026
Same author

Architectural principles of transporter-chaperone coupling within the native MHC I peptide-loading complex.

Science advances·2026

Related Experiment Video

Updated: Jun 23, 2025

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

6.9K

Engineering Mesoscale T Cell Receptor Clustering by Plug-and-Play Nanotools.

M Florencia Sánchez1, Sevi Faria1, Stefan Frühschulz1

  • 1Institute of Biochemistry, Biocenter, Goethe University Frankfurt, Max-von-Laue-Str. 9, 60438, Frankfurt am Main, Germany.

Advanced Materials (Deerfield Beach, Fla.)
|June 26, 2024
PubMed
Summary
This summary is machine-generated.

T cell receptor (TCR) clustering dynamics differ based on ligand presence. Peptide-loaded major histocompatibility complex (pMHC) induced clustering shows higher density and dynamics than ligand-free clustering.

Keywords:
immunoreceptors, membrane organizationmembrane protein patterningreceptor clusteringsignal transduction

More Related Videos

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

23.9K
Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
19:05

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

Published on: October 30, 2015

12.4K

Related Experiment Videos

Last Updated: Jun 23, 2025

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

6.9K
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

23.9K
Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
19:05

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

Published on: October 30, 2015

12.4K

Area of Science:

  • Immunology
  • Biophysics
  • Molecular Biology

Background:

  • T cell receptor (TCR) clustering and immune synapse formation are critical for TCR signaling.
  • Limited understanding exists regarding these dynamic assemblies and their link to transmembrane signaling.

Purpose of the Study:

  • To investigate T cell receptor (TCR) clustering using controlled nanotools.
  • To compare ligand-induced (pMHC) versus ligand-independent TCR assembly.
  • To explore how ligand size and architecture influence TCR clustering.

Main Methods:

  • Engineered nanotools with irreversible conjugation pairs and peptide-loaded major histocompatibility complex (pMHC) molecules were utilized.
  • Streptavidin-binding peptides facilitated anchoring in pre-structured matrices.
  • TCR clustering was analyzed in both ligand-induced and ligand-free conditions within confined regions.

Main Results:

  • pMHC-induced TCR clustering exhibited higher density and dynamics compared to ligand-free clustering.
  • The size and architecture of the pMHC ligand were found to influence TCR assembly dynamics.
  • Controlled manipulation of membrane receptor clustering was achieved with high specificity.

Conclusions:

  • Ligand engagement significantly impacts the density and dynamics of T cell receptor (TCR) clustering.
  • This platform allows for precise control over membrane receptor clustering.
  • The findings provide insights into diverse modes of T cell receptor activation.