Related Experiment Video
Updated: Jun 22, 2025
11:00
Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
Published on: January 12, 2015
11.5K
Systematic transcriptome profiling of hPSC-derived osteoblasts unveils CORIN's mastery in governing osteogenesis
Dandan Zhu1, Mo-Fan Huang2, An Xu1
1Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, Texas, USA.
The Journal of Biological Chemistry
|June 26, 2024
Summary
Researchers identified CORIN as a key protein promoting human osteoblast differentiation. Its depletion impairs bone formation, highlighting its crucial role in regulating bone development and the underlying CEBPD gene networks.
Area of Science:
- Biochemistry
- Cell Biology
- Genetics
Background:
- Human osteogenesis research is limited by available models and cell sources.
- Understanding the molecular mechanisms of human osteoblast differentiation is crucial for regenerative medicine and skeletal disease research.
Purpose of the Study:
- To systematically identify functional membrane proteins and transcriptional networks regulating human osteogenesis.
- To elucidate the role of CORIN and SDC1 in human osteoblast differentiation.
Main Methods:
- Utilized human pluripotent stem cell-derived osteoblasts for analysis.
- Performed functional assays, genome-wide chromatin immunoprecipitation (ChIP), and transcriptomic analyses (bulk and single-cell).
- Investigated the roles of CORIN, SDC1, CEBPD, and p38 MAPK signaling pathways.
Main Results:
- Identified CORIN as a human-specific transmembrane protease enriched in osteoblasts, essential for osteogenesis.
- Demonstrated that CORIN depletion significantly impairs osteoblast differentiation.
- Uncovered a mechanism where CORIN promotes osteogenesis via p38 MAPK-mediated upregulation of CCAAT enhancer binding protein delta (CEBPD).
- Showed SDC1 negatively regulates osteogenesis through a similar CEBPD-dependent pathway.
Conclusions:
- CORIN is a critical driver of human osteoblast lineage commitment.
- CORIN-mediated CEBPD transcriptomic networks are essential for human osteogenesis.
- Findings provide novel insights into the molecular regulation of human bone formation.
